Study of the Temperature and Molarity Ratio Effects in Geraniol Esterification and Testing Its Antibacterial Activity

Stefane Sugiarto, Novita Ariani, Elvina Dhiaful Iftitah, Galuh Widiyarti

2024

Abstract

This study modifies the molarity ratio of geraniol to isobutyric acid (1:1, 1:1.1, and 1:1.3) and temperature (RT, 40°C, 60°C, and 80°C) in the synthesis of geranyl isobutyrate ester using 5% (w/w) NaOH as a base catalyst. The antimicrobial activity was tested against both gram-positive and gram-negative bacteria. Ester products were separated and purified using column chromatography, and identified using Fourier Transform Infrared Spectroscopy (FTIR) and Gas Chromatography-Mass Spectrometry (GCMS). The antimicrobial activity was assessed using the disk diffusion method. The results showed that the esterification product with a 1:1.1 molar ratio at 80°C had the best separation based on thin-layer chromatography (TLC) and antimicrobial properties. GCMS analysis of the purified product revealed five compound peaks with geranyl isobutyrate at RT = 13.376 minutes (2.77%). FTIR confirmed the presence of C=O ester carbonyl groups at 1717.82 cm-1 and C-O groups at 1080.37 cm-1. Antimicrobial tests showed inhibition zones on gram-positive bacteria of 18.33±2.62 mm for Bacillus subtilis and 15.67±0.47 mm for Staphylococcus aureus, and against gram-negative bacteria of 10.67±0.47 mm for Pseudomonas aeruginosa and 16.67±2.36 mm for Escherichia coli.

Paper Citation

in Harvard Style

Sugiarto S., Ariani N., Iftitah E. and Widiyarti G. (2024). Study of the Temperature and Molarity Ratio Effects in Geraniol Esterification and Testing Its Antibacterial Activity. In Proceedings of BRIN’s 2nd International Conference for Health Research - Volume 1: ICHR; ISBN 978-989-758-755-9, SciTePress, pages 38-46. DOI: 10.5220/0013553900004612

in Bibtex Style

@conference{ichr24,
author={Stefane Sugiarto and Novita Ariani and Elvina Iftitah and Galuh Widiyarti},
title={Study of the Temperature and Molarity Ratio Effects in Geraniol Esterification and Testing Its Antibacterial Activity},
booktitle={Proceedings of BRIN’s 2nd International Conference for Health Research - Volume 1: ICHR},
year={2024},
pages={38-46},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0013553900004612},
isbn={978-989-758-755-9},
}

in EndNote Style

TY - CONF

JO - Proceedings of BRIN’s 2nd International Conference for Health Research - Volume 1: ICHR
TI - Study of the Temperature and Molarity Ratio Effects in Geraniol Esterification and Testing Its Antibacterial Activity
SN - 978-989-758-755-9
AU - Sugiarto S.
AU - Ariani N.
AU - Iftitah E.
AU - Widiyarti G.
PY - 2024
SP - 38
EP - 46
DO - 10.5220/0013553900004612
PB - SciTePress