Brine Shrimp Lethality Assay of Ethyl Cinnamate Derivatives Synthesized by Microwave Irradiation of Cinnamic Acids with Ethyl Acetate

Rose Malina Annur, Nisa Urribah, Lia Meilawati, Faris Hermawan, Salahuddin Salahuddin, Megawati Megawati, Megawati Megawati, Zetryana Puteri Tachrim

2024

Abstract

The simple and low-cost bioassay of brine shrimp lethality assay can be used to examine the toxicity effect of potential drugs. Ethyl p-methoxycinnamate is a major compound that can be isolated from the rhizome of Kaempferia galanga which was suggested that its ethyl ester is the most important functional group contributing to its bioactivity. In general, the synthesis of this typical ethyl ester of cinnamic acids is derived from the reaction of ethanol and cinnamic acid under reflux with the contribution of acid as a catalyst. In this study, cinnamic acid and selected para-substituted cinnamic acid derivatives are subjected to microwave irradiation-guided ethyl esterification by using ethyl acetate and sulfuric acid. This alternative method for ethyl esterification yielded 19–39% products of ethyl cinnamate derivatives and ethyl p-chloro cinnamate showed the highest toxicity level among the tested ethyl cinnamate derivatives with LC50 of 1.29 µg/mL.

Paper Citation

in Harvard Style

Annur R., Urribah N., Meilawati L., Hermawan F., Salahuddin S., Megawati M. and Tachrim Z. (2024). Brine Shrimp Lethality Assay of Ethyl Cinnamate Derivatives Synthesized by Microwave Irradiation of Cinnamic Acids with Ethyl Acetate. In Proceedings of BRIN’s 2nd International Conference for Health Research - Volume 1: ICHR; ISBN 978-989-758-755-9, SciTePress, pages 5-8. DOI: 10.5220/0013471800004612

in Bibtex Style

@conference{ichr24,
author={Rose Annur and Nisa Urribah and Lia Meilawati and Faris Hermawan and Salahuddin Salahuddin and Megawati Megawati and Zetryana Tachrim},
title={Brine Shrimp Lethality Assay of Ethyl Cinnamate Derivatives Synthesized by Microwave Irradiation of Cinnamic Acids with Ethyl Acetate},
booktitle={Proceedings of BRIN’s 2nd International Conference for Health Research - Volume 1: ICHR},
year={2024},
pages={5-8},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0013471800004612},
isbn={978-989-758-755-9},
}

in EndNote Style

TY - CONF

JO - Proceedings of BRIN’s 2nd International Conference for Health Research - Volume 1: ICHR
TI - Brine Shrimp Lethality Assay of Ethyl Cinnamate Derivatives Synthesized by Microwave Irradiation of Cinnamic Acids with Ethyl Acetate
SN - 978-989-758-755-9
AU - Annur R.
AU - Urribah N.
AU - Meilawati L.
AU - Hermawan F.
AU - Salahuddin S.
AU - Megawati M.
AU - Tachrim Z.
PY - 2024
SP - 5
EP - 8
DO - 10.5220/0013471800004612
PB - SciTePress