Endophytic Bacteria from White Cambodia Stems (Plumeria Acuminata) Have Strong Inhibition Against Escherichia Coli

Debie Rizqoh, Alvi Jalilul Hakim, Novriantika Lestari, Sipriyadi, Oktoviani

2023

Abstract

The health issues caused by Escherichia coli are a primary trigger for infectious diseases in Indonesia. Bacterial infections can be treated with antibiotics, leading to an increase in their usage. The rising use of antibiotics has led to irrational usage, resulting in the development of antibiotic-resistant bacteria. Therefore, there is a need for new antibiotic sources that can combat bacterial infections, especially from various biological resources. Due to its antimicrobial compound properties, white cambodia (Plumeria acuminata) is a biological resource with potential as an antibiotic. One way to utilize this potential is by isolating endophytic bacteria. The stem of the white cambodia provides a suitable environment for endophytic bacteria. Thus, this study aims to determine the antibacterial potential of endophytic bacterial isolates from the stem of white Cambodia to inhibit the growth of E. coli. This research uses a qualitative data collection method with laboratory experimental research. In the first stage, endophytic bacteria were isolated from the stem of P. acuminata using the serial dilution method. Subsequently, the characteristics of the colonies were observed based on their shape, edges, elevation, texture, pigmentation, and the result of Gram staining. In the final stage, the antagonistic test of the endophytic bacterial isolates against E. coli was conducted using the double-layer agar method. The isolation of endophytic bacteria from P. acuminata resulted in 3,817 colonies. Based on the observation of colony morphology, 93 isolates have different colony morphologies due to the diverse characteristics of the colonies and bacterial shapes. The Gram staining test showed that 83 endophytic bacterial isolates were Gram-positive, and ten were Gram-negative. The antagonistic test revealed that seven positive endophytic bacterial isolates could inhibit the growth of E. coli. Endophytic bacterial isolates from P. acuminata can produce antibacterial compounds that can inhibit the growth of E. coli.

Paper Citation

in Harvard Style

Rizqoh D., Hakim A., Lestari N., Sipriyadi. and Oktoviani. (2023). Endophytic Bacteria from White Cambodia Stems (Plumeria Acuminata) Have Strong Inhibition Against Escherichia Coli. In Proceedings of the 1st International Conference on Medical Science and Health - Volume 1: ICOMESH; ISBN 978-989-758-740-5, SciTePress, pages 303-310. DOI: 10.5220/0013671700003873

in Bibtex Style

@conference{icomesh23,
author={Debie Rizqoh and Alvi Jalilul Hakim and Novriantika Lestari and Sipriyadi and Oktoviani},
title={Endophytic Bacteria from White Cambodia Stems (Plumeria Acuminata) Have Strong Inhibition Against Escherichia Coli},
booktitle={Proceedings of the 1st International Conference on Medical Science and Health - Volume 1: ICOMESH},
year={2023},
pages={303-310},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0013671700003873},
isbn={978-989-758-740-5},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 1st International Conference on Medical Science and Health - Volume 1: ICOMESH
TI - Endophytic Bacteria from White Cambodia Stems (Plumeria Acuminata) Have Strong Inhibition Against Escherichia Coli
SN - 978-989-758-740-5
AU - Rizqoh D.
AU - Hakim A.
AU - Lestari N.
AU - Sipriyadi.
AU - Oktoviani.
PY - 2023
SP - 303
EP - 310
DO - 10.5220/0013671700003873
PB - SciTePress