Novel Primers for Easy Detection of Yellow Fever Using Isothermal PCR

Naufal Dimas Lingga Takbirrahman, Dea Syafira Alamsyah Sitompul, Nicholas Gabriel Harefa, Aref Budi Witarto

2023

Abstract

Yellow Fever is a disease caused by the Yellow Fever Virus (YFV), common in tropical areas in Africa and South America. Hundreds of Indonesian soldiers are sent on peacekeeping missions to Congo -an endemic region for YFV- every year. Therefore, an accurate technique is needed to screen those who possibly get infections. Isothermal PCR, unlike conventional PCR, uses a single temperature with 4 to 6 combinations of primers that can be performed with simple equipment such as a heat block and detected with a visual eye instead of agarose electrophoresis. Thus, it is suitable for this challenge. YFV strain 17D genome was used and particularly NS2a and NS5 genes were chosen as template for the design of primers. To check for specificity, obtained primers were checked for sequence similarity using BLASTN. Literature studies showed that various genes, including E and NS5, have been used for the target using conventional PCR. Meanwhile, for isothermal PCR, only the NS1 gene and upstream non-coding gene have been used. From the NS2a gene with length of 672 bp, 2 sets of primers were obtained. At the same time, NS5, with a longer length of 2,714 bp, gave 4 sets of primers. BLASTN results showed that all primers were specific to the YFV genome. Original primers have been successfully designed for isothermal PCR of yellow fever. Primers designed are the first step and also the most critical original works in nucleic acid testing. Therefore, towards self-sufficient diagnostic reagents, the results are significant.

Paper Citation

in Harvard Style

Takbirrahman N., Sitompul D., Harefa N. and Witarto A. (2023). Novel Primers for Easy Detection of Yellow Fever Using Isothermal PCR. In Proceedings of the 1st International Conference on Medical Science and Health - Volume 1: ICOMESH; ISBN 978-989-758-740-5, SciTePress, pages 299-302. DOI: 10.5220/0013671600003873

in Bibtex Style

@conference{icomesh23,
author={Naufal Dimas Lingga Takbirrahman and Dea Syafira Alamsyah Sitompul and Nicholas Gabriel Harefa and Aref Budi Witarto},
title={Novel Primers for Easy Detection of Yellow Fever Using Isothermal PCR},
booktitle={Proceedings of the 1st International Conference on Medical Science and Health - Volume 1: ICOMESH},
year={2023},
pages={299-302},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0013671600003873},
isbn={978-989-758-740-5},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 1st International Conference on Medical Science and Health - Volume 1: ICOMESH
TI - Novel Primers for Easy Detection of Yellow Fever Using Isothermal PCR
SN - 978-989-758-740-5
AU - Takbirrahman N.
AU - Sitompul D.
AU - Harefa N.
AU - Witarto A.
PY - 2023
SP - 299
EP - 302
DO - 10.5220/0013671600003873
PB - SciTePress