Physicochemical Analyses and Antibacterial Potential of Propolis by Stingless Bee (Homotrigona apicalis) Found in East Kalimantan, Indonesia

Supomo, Supomo, Enos Tangke Arung, Irawan Wijaya Kusuma, Dewi Sondari

2023

Abstract

This study examined the antibacterial potential of Homotrigona apicalis propolis extract against Staphylococcus epidermidis and Propionibacterium acnes, which are bacteria found on human skin that can cause opportunistic infections. The extract was obtained through the collection, processing, extraction, and fractionation of fresh herb samples. The antibacterial activity of the methanol extract, n-hexane fraction, ethylacetate fraction and residual fraction, at concentrations of 2.5%, 5%, and 10% was evaluated using the disc diffusion method, with clindamycin 0,1% and DMSO 1% as positive and negative controls respectively. Statistical analysis was performed using ANOVA. The results revealed that both the methanol extracts and the ethyl acetate fraction of Homotrigona apicalis propolis showed significant inhibitory effects on bacterial growth. The greatest antibacterial activity was observed at the concentration ethyl acetate fraction of 10%, with inhibition zones of 13.27 mm for Staphylococcus epidermidis and 12.86 mm for Propionibacterium acnes. Importantly, Staphylococcus epidermidis exhibited higher susceptibility to the propolis extract and fraction compared to Propionibacterium acnes. These findings suggest that Homotrigona apicalis propolis extract has the potential to be used as an active ingredient in cosmetics targeting acne-causing bacteria, specifically Staphylococcus epidermidis and Propionibacterium acnes. The extract demonstrated efficacy against these pathogens suggests its potential as an alternative to conventional antibiotics.

Paper Citation

in Harvard Style

Supomo., Arung E., Kusuma I. and Sondari D. (2023). Physicochemical Analyses and Antibacterial Potential of Propolis by Stingless Bee (Homotrigona apicalis) Found in East Kalimantan, Indonesia. In Proceedings of the 1st International Conference on Medical Science and Health - Volume 1: ICOMESH; ISBN 978-989-758-740-5, SciTePress, pages 143-150. DOI: 10.5220/0013667100003873

in Bibtex Style

@conference{icomesh23,
author={Supomo and Enos Tangke Arung and Irawan Wijaya Kusuma and Dewi Sondari},
title={Physicochemical Analyses and Antibacterial Potential of Propolis by Stingless Bee (Homotrigona apicalis) Found in East Kalimantan, Indonesia},
booktitle={Proceedings of the 1st International Conference on Medical Science and Health - Volume 1: ICOMESH},
year={2023},
pages={143-150},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0013667100003873},
isbn={978-989-758-740-5},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 1st International Conference on Medical Science and Health - Volume 1: ICOMESH
TI - Physicochemical Analyses and Antibacterial Potential of Propolis by Stingless Bee (Homotrigona apicalis) Found in East Kalimantan, Indonesia
SN - 978-989-758-740-5
AU - Supomo.
AU - Arung E.
AU - Kusuma I.
AU - Sondari D.
PY - 2023
SP - 143
EP - 150
DO - 10.5220/0013667100003873
PB - SciTePress