Effects of Skeletonema Costatum’s Powder on the Knee Joint’s Diameter and the Degree of Pain of Male Rat Sparague Dawley Type Induced by Complete Freund’s Adjuvant

Astalitha Lorel Tania, Riana Rahmawati, Isnatin Miladiyah

2023

Abstract

Osteoarthritis is a degenerative disease caused by an abnormal inflammatory cytokine response. Complete Freund's Adjuvant (CFA) is a chemical substance that induces osteoarthritis. Bioactive compounds in Skeletonema costatum act as antioxidants, which could lessen inflammation. This study aims to determine how Skeletonema costatum affects the knee joint's diameter size and the degree of pain induced by CFA in rats. The study used thirty adult male rats (Sparague dawley), 2-3 months old, weighing 200-300 grams, divided into five groups: negative control (K1), positive control/piroxicam (K2), and intervention groups (K1, K2, and K3). All groups were induced with CFA, and the intervention began six weeks after induction. The negative and positive groups received normal saline and piroxicam (10 mg/kg BW/day) for 21 days, respectively. Skeletonema costatum was given to the intervention groups at doses of 60 mg/kg BW/day (K3), 90 mg/kg BW/day (K4), and 120 mg/kg BW/day (K5) for 21 days. The knee joint diameters and the degree of pain were assessed using a micrometer screw and a hot water tail-flick assay on days 0, 7, 14, and 21. The results were examined using the paired-t test, Kruskall-Wallis, and post hoc Mann-Whitney. Skeletonema costatum lowered the diameters and pain levels of rats' knee joints before and after the trial (paired t-test, p<0.05). All dosage groups demonstrated a beneficial effect (Kruskal Wallis, p<0.05); the higher the dose of Skeletonema costatum, the greater the effect on reducing the knee joint's diameter and pain. A multivariate analysis showed the reduction of knee joint diameter and pain after Skeletonema costatum's intervention was statistically greater compared to the control/placebo group. However, this effect was lower than in the piroxicam group (p< 0.05). Skeletonema costatum may have an anti-inflammatory effect in rats by lowering the size of the knee joints and the degree of pain induced by CFA. Given the potential of Indonesian marine products and the trend toward marine drug treatments, further studies are required to investigate the inflammatory effects of Skeletonema costatum.

Paper Citation

in Harvard Style

Tania A., Rahmawati R. and Miladiyah I. (2023). Effects of Skeletonema Costatum’s Powder on the Knee Joint’s Diameter and the Degree of Pain of Male Rat Sparague Dawley Type Induced by Complete Freund’s Adjuvant. In Proceedings of the 1st International Conference on Medical Science and Health - Volume 1: ICOMESH; ISBN 978-989-758-740-5, SciTePress, pages 5-10. DOI: 10.5220/0013084800003873

in Bibtex Style

@conference{icomesh23,
author={Astalitha Lorel Tania and Riana Rahmawati and Isnatin Miladiyah},
title={Effects of Skeletonema Costatum’s Powder on the Knee Joint’s Diameter and the Degree of Pain of Male Rat Sparague Dawley Type Induced by Complete Freund’s Adjuvant},
booktitle={Proceedings of the 1st International Conference on Medical Science and Health - Volume 1: ICOMESH},
year={2023},
pages={5-10},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0013084800003873},
isbn={978-989-758-740-5},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 1st International Conference on Medical Science and Health - Volume 1: ICOMESH
TI - Effects of Skeletonema Costatum’s Powder on the Knee Joint’s Diameter and the Degree of Pain of Male Rat Sparague Dawley Type Induced by Complete Freund’s Adjuvant
SN - 978-989-758-740-5
AU - Tania A.
AU - Rahmawati R.
AU - Miladiyah I.
PY - 2023
SP - 5
EP - 10
DO - 10.5220/0013084800003873
PB - SciTePress