Ultra-Sensitivity Widefield, Confocal Surface Plasmon Interferometry Using Sequential Coding

Suejit Pechprasarn

2023

Abstract

Interferometry has been a standard technique for optical phase measurement. Most single-molecule sensitivity measurements and imaging tools rely very much on the interferometric measurement of dual optical beams. We have developed an embedded confocal interferometric microscope and demonstrated by theoretical calculation that the system can achieve single molecule detection sensitivity. Of course, several challenges need to be addressed to achieve such ultra-sensitivity. The confocal surface plasmon microscope is a beam scanning system, and it also suffers from thermal variations due to long data acquisition time. Here we propose a widefield quantitative confocal surface plasmon interferometric microscope configuration using orthogonal coding. The proposed system sequentially illuminates a plasmonic sample with multiple focal points in the sample plane based on an orthogonal code such as a Hadamard code. The images of the illumination sequences are then captured and processed with the known Hadamard input sequence. Here we show that employing the Hadamard time coding in the confocal surface plasmon interferometry enables us to (1) perform widefield imaging, (2) higher signal-to-noise compared to the beam scanning system, (3) high sensitivity, (4) good spatial resolution and (5) more stable compared to the confocal surface plasmon microscope.

Paper Citation

in Harvard Style

Pechprasarn S. (2023). Ultra-Sensitivity Widefield, Confocal Surface Plasmon Interferometry Using Sequential Coding. In Proceedings of the 11th International Conference on Photonics, Optics and Laser Technology - Volume 1: PHOTOPTICS, ISBN 978-989-758-632-3, pages 96-101. DOI: 10.5220/0011892300003408

in Bibtex Style

@conference{photoptics23,
author={Suejit Pechprasarn},
title={Ultra-Sensitivity Widefield, Confocal Surface Plasmon Interferometry Using Sequential Coding},
booktitle={Proceedings of the 11th International Conference on Photonics, Optics and Laser Technology - Volume 1: PHOTOPTICS,},
year={2023},
pages={96-101},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0011892300003408},
isbn={978-989-758-632-3},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 11th International Conference on Photonics, Optics and Laser Technology - Volume 1: PHOTOPTICS,
TI - Ultra-Sensitivity Widefield, Confocal Surface Plasmon Interferometry Using Sequential Coding
SN - 978-989-758-632-3
AU - Pechprasarn S.
PY - 2023
SP - 96
EP - 101
DO - 10.5220/0011892300003408