Measurement of Platelet Aggregation in Ageing Samples and After in-Vitro Activation

Christian Klenk, David Fresacher, David Fresacher, Stefan Röhrl, Dominik Heim, Manuel Lengl, Simon Schumann, Martin Knopp, Klaus Diepold, Stefan Holdenrieder, Oliver Hayden

2023

Abstract

Blood cell aggregates are gaining importance as a possible biomarker for various diseases. However, due to technical limitations of common analysers, mostly only interactions between leukocytes and platelets are measured directly as aggregates. Interactions between platelets are usually only measured indirectly after using an activation assay or by analysing surface proteins. Here, an imaging flow cytometer is used to measure and characterize platelet-aggregates directly in whole blood samples. Influences of sample ageing and in-vitro activation with adenosine diphosphate (ADP) was investigated for blood anticoagulated with either EDTA, citrate, heparin or hirudin. Here, the number of platelet-aggregates and their composition was measured. Blood anticoagulated with hirudin and EDTA showed a stable number of aggregates within a timeframe of 240 minutes. While no aggregate concentration changes were observed in EDTA blood after activation with ADP, a clear increase in aggregates was seen in hirudin, citrate and heparin blood. This effect is also observable when looking at the composition of the clots. However, after an initial spike a large number of aggregates disintegrate within a time frame of nine minutes. This effect is particularly prominent for large aggregates containing six or more platelets.

Paper Citation

in Harvard Style

Klenk C., Fresacher D., Röhrl S., Heim D., Lengl M., Schumann S., Knopp M., Diepold K., Holdenrieder S. and Hayden O. (2023). Measurement of Platelet Aggregation in Ageing Samples and After in-Vitro Activation. In Proceedings of the 16th International Joint Conference on Biomedical Engineering Systems and Technologies (BIOSTEC 2023) - Volume 2: BIOIMAGING; ISBN 978-989-758-631-6, SciTePress, pages 57-65. DOI: 10.5220/0011634200003414

in Bibtex Style

@conference{bioimaging23,
author={Christian Klenk and David Fresacher and Stefan Röhrl and Dominik Heim and Manuel Lengl and Simon Schumann and Martin Knopp and Klaus Diepold and Stefan Holdenrieder and Oliver Hayden},
title={Measurement of Platelet Aggregation in Ageing Samples and After in-Vitro Activation},
booktitle={Proceedings of the 16th International Joint Conference on Biomedical Engineering Systems and Technologies (BIOSTEC 2023) - Volume 2: BIOIMAGING},
year={2023},
pages={57-65},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0011634200003414},
isbn={978-989-758-631-6},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 16th International Joint Conference on Biomedical Engineering Systems and Technologies (BIOSTEC 2023) - Volume 2: BIOIMAGING
TI - Measurement of Platelet Aggregation in Ageing Samples and After in-Vitro Activation
SN - 978-989-758-631-6
AU - Klenk C.
AU - Fresacher D.
AU - Röhrl S.
AU - Heim D.
AU - Lengl M.
AU - Schumann S.
AU - Knopp M.
AU - Diepold K.
AU - Holdenrieder S.
AU - Hayden O.
PY - 2023
SP - 57
EP - 65
DO - 10.5220/0011634200003414
PB - SciTePress