Selection of Aflatoxin B1 Mimic Epitope Peptides by Phage Display

Ke Wu, Xiaoyan Qiu, Renrong Liu

2022

Abstract

Aflatoxin B1 (AFB1) is a natural pollutant with strong toxicity and carcinogenicity. It not only causes huge economic losses, but also poses a serious threat to human, livestock and poultry health. To acquire the mimic epitope peptide of aflatoxin B1 and establish a non-toxic detection system of aflatoxin, an anti-afb13c7 monoclonal antibody was used as the target molecule, and the mimic epitopes of AFB1 were screened from the phage random 7 peptide library. A total of 39 phage clones were selected for verification. 36 of them could specifically bind to the antibody, and 30 of them could be inhibited by AFB1, whose DNA was extracted and sequenced. The results showed that 30 positive phage particles were actually 17 phage clones. The common sequence was histidine (H) - proline (P) - tryptophan (W), abbreviated as xxxxhpw, xxxhpwx, xxhpwxx, xhpwxxx (X was any amino acid). The linear range, detection limit and half inhibitory concentration (IC50) of the 17 positive phage particles were similar. The linear range was 1-2000 pg / ml.

Paper Citation

in Harvard Style

Wu K., Qiu X. and Liu R. (2022). Selection of Aflatoxin B1 Mimic Epitope Peptides by Phage Display. In Proceedings of the 4th International Conference on Biomedical Engineering and Bioinformatics - Volume 1: ICBEB, ISBN 978-989-758-595-1, pages 1279-1283. DOI: 10.5220/0011508600003443

in Bibtex Style

@conference{icbeb22,
author={Ke Wu and Xiaoyan Qiu and Renrong Liu},
title={Selection of Aflatoxin B1 Mimic Epitope Peptides by Phage Display},
booktitle={Proceedings of the 4th International Conference on Biomedical Engineering and Bioinformatics - Volume 1: ICBEB,},
year={2022},
pages={1279-1283},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0011508600003443},
isbn={978-989-758-595-1},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 4th International Conference on Biomedical Engineering and Bioinformatics - Volume 1: ICBEB,
TI - Selection of Aflatoxin B1 Mimic Epitope Peptides by Phage Display
SN - 978-989-758-595-1
AU - Wu K.
AU - Qiu X.
AU - Liu R.
PY - 2022
SP - 1279
EP - 1283
DO - 10.5220/0011508600003443