Extraction of Intrinsic Fluorescence in Fluorescence Imaging of Turbid Tissues

Gennadi Saiko, Alexandre Douplik

Abstract

Interpretation of tissue fluorescence spectra can be complicated due to interplay with tissue optics. We have developed a photon propagation approach for correction of fluorescence on absorption in two realistic scenarios: when fluorophores are located a) on the surface of the turbid tissue and b) in a layer inside the turbid tissue. The approach takes into account the diffuse reflection of the tissue at excitation and emission wavelengths and does not require any precise measurement of optical properties (e.g., coefficient of absorption). The approach can be implemented using an inexpensive imaging setup and can be used in any setting.

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Paper Citation


in Harvard Style

Saiko G. and Douplik A. (2020). Extraction of Intrinsic Fluorescence in Fluorescence Imaging of Turbid Tissues.In Proceedings of the 13th International Joint Conference on Biomedical Engineering Systems and Technologies - Volume 2: BIOIMAGING, ISBN 978-989-758-398-8, pages 123-129. DOI: 10.5220/0008919401230129


in Bibtex Style

@conference{bioimaging20,
author={Gennadi Saiko and Alexandre Douplik},
title={Extraction of Intrinsic Fluorescence in Fluorescence Imaging of Turbid Tissues},
booktitle={Proceedings of the 13th International Joint Conference on Biomedical Engineering Systems and Technologies - Volume 2: BIOIMAGING,},
year={2020},
pages={123-129},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0008919401230129},
isbn={978-989-758-398-8},
}


in EndNote Style

TY - CONF

JO - Proceedings of the 13th International Joint Conference on Biomedical Engineering Systems and Technologies - Volume 2: BIOIMAGING,
TI - Extraction of Intrinsic Fluorescence in Fluorescence Imaging of Turbid Tissues
SN - 978-989-758-398-8
AU - Saiko G.
AU - Douplik A.
PY - 2020
SP - 123
EP - 129
DO - 10.5220/0008919401230129