The Sensitivity Evaluation of mt-DNA Genes; NADH Dehydrogenase Sub Unit 5 (ND5), D-Loop, and Cytochrome-b (Cty-b) to Detect Pork (Sus scrofa) DNA Isolate and DNA Fragment in Meatball using PCR Technique

Joni Kusnadi, Joni Kusnadi, Noval Ashari

Abstract

The utilization of mt-DNA primers on previous study specific to detect DNA pork fragments. This study aims were to evaluate the sensitivity of mt-DNA primers (ND5, D-Loop, and Cyt-b) in pork DNA isolates and its meatballs product. The sensitivity analysis was conducted in pork DNA isolates with concentrations (10, 1, 10-1, 10-2, 10-3, 10-4, 10-5, and 10-6 ng/µl) and its meatballs product with variation of pork content (0%, 0.01%, 0.05%, 0.1%, 0.5%, 1%, and 5%). Furthermore, the DNA fragment amplification process was carried out using PCR technique. The amplification results showed that ND5 and Cyt-b primers were more sensitive because they were able to amplify DNA with concentrations up to 10-3 ng/µl compared to D-Loop which was only able to amplify with concentrations up to 10-2 ng/µl. The sensitivity results using meatballs showed that ND5 was the most sensitive primer in detecting meatballs with concentration of pork up to 0.01%. It can be concluded that ND5, Cyt-b, and D-Loop primers are able to detect pork DNA fragment with high sensitivity. The ND5 primer gave the most sensitive amplification results because it was able to detect pork DNA fragments with the lowest concentration and meatball with the lowest pork content.

Download


Paper Citation


in Harvard Style

Kusnadi J. and Ashari N. (2020). The Sensitivity Evaluation of mt-DNA Genes; NADH Dehydrogenase Sub Unit 5 (ND5), D-Loop, and Cytochrome-b (Cty-b) to Detect Pork (Sus scrofa) DNA Isolate and DNA Fragment in Meatball using PCR Technique. In Proceedings of the 6th Food Ingredient Asia Conference - Volume 1: 6th FiAC, ISBN 978-989-758-540-1, pages 12-19. DOI: 10.5220/0010507000003108


in Bibtex Style

@conference{6th fiac20,
author={Joni Kusnadi and Noval Ashari},
title={The Sensitivity Evaluation of mt-DNA Genes; NADH Dehydrogenase Sub Unit 5 (ND5), D-Loop, and Cytochrome-b (Cty-b) to Detect Pork (Sus scrofa) DNA Isolate and DNA Fragment in Meatball using PCR Technique},
booktitle={Proceedings of the 6th Food Ingredient Asia Conference - Volume 1: 6th FiAC,},
year={2020},
pages={12-19},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0010507000003108},
isbn={978-989-758-540-1},
}


in EndNote Style

TY - CONF

JO - Proceedings of the 6th Food Ingredient Asia Conference - Volume 1: 6th FiAC,
TI - The Sensitivity Evaluation of mt-DNA Genes; NADH Dehydrogenase Sub Unit 5 (ND5), D-Loop, and Cytochrome-b (Cty-b) to Detect Pork (Sus scrofa) DNA Isolate and DNA Fragment in Meatball using PCR Technique
SN - 978-989-758-540-1
AU - Kusnadi J.
AU - Ashari N.
PY - 2020
SP - 12
EP - 19
DO - 10.5220/0010507000003108