Antibacterial Activity of Synthesized Silver Nanoparticle using Langsat Leaf Extract (Lansium domesticum var. pubescen Kooders et Valeton) as Bioreductor against Escherichia coli and Staphylococcus aureus

Khairunnida Rahma, Agung Dwi Wahyu Widodo, Rebekah Juniati Setiabudi, Retno Indrawati Roestamadji, Maftuchah Rochmanti, Pudji Lestari

Abstract

The emerging of antimicrobial resistance has caused the urgency to find new alternative agents. The application of silver in the form of silver nanoparticles (AgNP) began to be studied again. The less toxic and ecofriendly method to synthesize AgNP is through the green synthesis approach using plant extract. Langsat is one of the endemic plants of South Kalimantan and its leaf provides secondary metabolites substances to help the synthesize of AgNP. The synthesis of AgNP was prepared and AgNO3 using Langsat Leaf (LL) extract as the bioreductor. The synthesized LL-AgNP was then characterized by UV-Vis spectrophotometer on 575 nm. The MIC study was done using broth dilution method with 6 different concentrations ranged from 3.125% to 100% and 2 controls, followed by the MBC study on MHA plates. The LL-AgNP successfully inhibited the growth of Escherichia coli and Staphylococcus aureus in concentration of 6.25% and 25%, respectively. The LL-AgNP also showed bactericidal activity against Escherichia coli in concentration of 25% but showed no activity on Staphylococcus aureus. This result indicates that LL-AgNP has potential as an antibacterial agent against Escherichia coli and Staphylococcus aureus.

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in Harvard Style

Rahma K., Wahyu Widodo A., Setiabudi R., Roestamadji R., Rochmanti M. and Lestari P. (2020). Antibacterial Activity of Synthesized Silver Nanoparticle using Langsat Leaf Extract (Lansium domesticum var. pubescen Kooders et Valeton) as Bioreductor against Escherichia coli and Staphylococcus aureus.In Proceedings of the 1st Jenderal Soedirman International Medical Conference in conjunction with the 5th Annual Scientific Meeting (Temilnas) Consortium of Biomedical Science Indonesia - Volume 1: JIMC, ISBN 978-989-758-499-2, pages 298-304. DOI: 10.5220/0010491702980304


in Bibtex Style

@conference{jimc20,
author={Khairunnida Rahma and Agung Dwi Wahyu Widodo and Rebekah Juniati Setiabudi and Retno Indrawati Roestamadji and Maftuchah Rochmanti and Pudji Lestari},
title={Antibacterial Activity of Synthesized Silver Nanoparticle using Langsat Leaf Extract (Lansium domesticum var. pubescen Kooders et Valeton) as Bioreductor against Escherichia coli and Staphylococcus aureus},
booktitle={Proceedings of the 1st Jenderal Soedirman International Medical Conference in conjunction with the 5th Annual Scientific Meeting (Temilnas) Consortium of Biomedical Science Indonesia - Volume 1: JIMC,},
year={2020},
pages={298-304},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0010491702980304},
isbn={978-989-758-499-2},
}


in EndNote Style

TY - CONF

JO - Proceedings of the 1st Jenderal Soedirman International Medical Conference in conjunction with the 5th Annual Scientific Meeting (Temilnas) Consortium of Biomedical Science Indonesia - Volume 1: JIMC,
TI - Antibacterial Activity of Synthesized Silver Nanoparticle using Langsat Leaf Extract (Lansium domesticum var. pubescen Kooders et Valeton) as Bioreductor against Escherichia coli and Staphylococcus aureus
SN - 978-989-758-499-2
AU - Rahma K.
AU - Wahyu Widodo A.
AU - Setiabudi R.
AU - Roestamadji R.
AU - Rochmanti M.
AU - Lestari P.
PY - 2020
SP - 298
EP - 304
DO - 10.5220/0010491702980304