Expression of MicroRNA-155 and Suppressor of Cytokines Signaling 1 (SOCS1) mRNA in Plasma Breast Cancer Patients

Dwi Nur Indah Sari, Dina Rahmina, Sumadi Lukman, Artanto Wahyono, Indwiani Astuti, Sofia Mubarika, Teguh Aryandono

Abstract

Breast cancer is the most common cancer in women worldwide. miR-155 has been discovered to have an important role in the development of breast cancer. miR-155 targets many mRNAs of tumor suppressor genes, one of them is SOCS1. The discovery of miRNA in body fluids provides a novel alternative for the development of minimally invasive biomarker in cancer. However, limited study has been reported on the expression of miR-155 and SOCS1 mRNA in plasma breast cancer patients especially in Indonesian population.This study analyzed expressions of miR-155 and SOCS1 mRNA in plasma breast cancer patients This study was conducted using cross-sectional design, 32 plasma samples were collected from Dr.Sardjito Hospital. RNA was extracted from plasma and then cDNA was synthesized from RNA samples. Real-time qPCR was used to detect expression of miR-155 and mRNA SOCS1. Both expression was analyzed using Livak's method. The significance of the results were tested statistically using independence T-test. Expression of miR-155 at advanced stage was 2.43 higher than the early stages (p=0.047), while the mRNA expression of SOCS1 at advanced stage was 1.29 lower than early stage (p=0.170). Expression of miR-155 in ER, PR, & HER2 status revealed differences among subgroups, while the mRNA expression of SOCS1 was not significantly different. Other result showed that miR-155 expression was higher in triple negative tumor, while SOCS1 higher in luminal A tumor. Nevertheless, the difference was also not statistically significant (p value > 0,05). This study showed that expressions of miR-155 and SOCS1 mRNA was deregulated in breast cancer plasma and it believed to have an important role in breast cancer.

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Paper Citation


in Harvard Style

Indah Sari D., Rahmina D., Lukman S., Wahyono A., Astuti I., Mubarika S. and Aryandono T. (2020). Expression of MicroRNA-155 and Suppressor of Cytokines Signaling 1 (SOCS1) mRNA in Plasma Breast Cancer Patients.In Proceedings of the 1st Jenderal Soedirman International Medical Conference in conjunction with the 5th Annual Scientific Meeting (Temilnas) Consortium of Biomedical Science Indonesia - Volume 1: JIMC, ISBN 978-989-758-499-2, pages 223-230. DOI: 10.5220/0010490402230230


in Bibtex Style

@conference{jimc20,
author={Dwi Nur Indah Sari and Dina Rahmina and Sumadi Lukman and Artanto Wahyono and Indwiani Astuti and Sofia Mubarika and Teguh Aryandono},
title={Expression of MicroRNA-155 and Suppressor of Cytokines Signaling 1 (SOCS1) mRNA in Plasma Breast Cancer Patients},
booktitle={Proceedings of the 1st Jenderal Soedirman International Medical Conference in conjunction with the 5th Annual Scientific Meeting (Temilnas) Consortium of Biomedical Science Indonesia - Volume 1: JIMC,},
year={2020},
pages={223-230},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0010490402230230},
isbn={978-989-758-499-2},
}


in EndNote Style

TY - CONF

JO - Proceedings of the 1st Jenderal Soedirman International Medical Conference in conjunction with the 5th Annual Scientific Meeting (Temilnas) Consortium of Biomedical Science Indonesia - Volume 1: JIMC,
TI - Expression of MicroRNA-155 and Suppressor of Cytokines Signaling 1 (SOCS1) mRNA in Plasma Breast Cancer Patients
SN - 978-989-758-499-2
AU - Indah Sari D.
AU - Rahmina D.
AU - Lukman S.
AU - Wahyono A.
AU - Astuti I.
AU - Mubarika S.
AU - Aryandono T.
PY - 2020
SP - 223
EP - 230
DO - 10.5220/0010490402230230