Dose-dependent Decaffeinated Green Tea Extract Administration Improved Hyperglycemia through Modulation of IRS-1 and GLUT-4 Genes Expression in Metabolic Syndrome Rat Model

Dwi Adi Nugroho, Mifetika Lukitasari, Marlita Marlita, Mohammad Saifur Rohman, Nashi Widodo, Inggita Kusumastuty, Nur Ida Panca Nugrahini

Abstract

Hyperglycemia is a major disorder in metabolic syndrome. Skeletal IRS-1 dan GLUT-4 expression are the key target in hyperglycemia improvement. This study aimed to investigate green tea extract's effect on hyperglycemia improvement in metabolic syndrome rat models. Twenty Sprague Dawley Metabolic Syndrome Rat Model weighed 300 – 400 grams were divided into GTE 200 (n=5) and GTE 400 (n=5) groups. Moreover, as control groups, ten rats were divided into normal control (NC) (n=5) and metabolic syndrome (MS) (n=5) groups. Rats in the GTE 200 and 400 groups were treated once daily with green tea extract at a dose of 200 and 400 mg/bw.t, respectively. The extract was administered for 9 weeks through oral gavage. RT-PCR methods analyzed skeletal IRS-1 and GLUT-4 gene expression. This study showed that the fasting blood glucose of GTE 200 dan GTE 400 was significantly lower than those of the MS group (p<0.001 and p<0.001, respectively). In addition, GTE 400 group had the lowest fasting blood glucose. Moreover, skeletal IRS-1 dan GLUT-4 gene expression was significantly higher in the GTE 200 and GTE 400 group than those of the MS group. In contrast, the GTE 400 group's gene expression was the highest among all groups (p<0.000 and p<0.002, respectively). Administration of green tea extract improved hyperglycemia in a metabolic syndrome rat model in a dose-dependent manner through skeletal IRS-1 dan GLUT-4 gene expression modulation.

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in Harvard Style

Nugroho D., Lukitasari M., Marlita M., Rohman M., Widodo N., Kusumastuty I. and Panca Nugrahini N. (2020). Dose-dependent Decaffeinated Green Tea Extract Administration Improved Hyperglycemia through Modulation of IRS-1 and GLUT-4 Genes Expression in Metabolic Syndrome Rat Model.In Proceedings of the 1st Jenderal Soedirman International Medical Conference in conjunction with the 5th Annual Scientific Meeting (Temilnas) Consortium of Biomedical Science Indonesia - Volume 1: JIMC, ISBN 978-989-758-499-2, pages 69-74. DOI: 10.5220/0010487900690074


in Bibtex Style

@conference{jimc20,
author={Dwi Adi Nugroho and Mifetika Lukitasari and Marlita Marlita and Mohammad Saifur Rohman and Nashi Widodo and Inggita Kusumastuty and Nur Ida Panca Nugrahini},
title={Dose-dependent Decaffeinated Green Tea Extract Administration Improved Hyperglycemia through Modulation of IRS-1 and GLUT-4 Genes Expression in Metabolic Syndrome Rat Model},
booktitle={Proceedings of the 1st Jenderal Soedirman International Medical Conference in conjunction with the 5th Annual Scientific Meeting (Temilnas) Consortium of Biomedical Science Indonesia - Volume 1: JIMC,},
year={2020},
pages={69-74},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0010487900690074},
isbn={978-989-758-499-2},
}


in EndNote Style

TY - CONF

JO - Proceedings of the 1st Jenderal Soedirman International Medical Conference in conjunction with the 5th Annual Scientific Meeting (Temilnas) Consortium of Biomedical Science Indonesia - Volume 1: JIMC,
TI - Dose-dependent Decaffeinated Green Tea Extract Administration Improved Hyperglycemia through Modulation of IRS-1 and GLUT-4 Genes Expression in Metabolic Syndrome Rat Model
SN - 978-989-758-499-2
AU - Nugroho D.
AU - Lukitasari M.
AU - Marlita M.
AU - Rohman M.
AU - Widodo N.
AU - Kusumastuty I.
AU - Panca Nugrahini N.
PY - 2020
SP - 69
EP - 74
DO - 10.5220/0010487900690074