The Effectivity of Butanol Fraction of Calophyllum nodosum as Antiviral Drug to Dengue Virus Serotype 2 In Vitro

Syifa Salsabila, Nabilla Calista, Hidayati Desti, Beti Ernawati Dewi

Abstract

Dengue fever still has a high incidence rate especially in Indonesia. Until now, there is no dengue antiviral therapy found. Researches to develop dengue antiviral from herbal sources had been done. One of the potential plants as dengue antiviral is Calophyllum nodosum which is known to have antimicrobial activity. This study to evaluate the antiviral effects of butanol fraction of Calophyllum nodosum on DENV-2 activity with Huh-7-it cells as host cells and also to evaluate minimal inhibitory concentration. Antiviral capability was measured by 50% cytotoxic concentration (CC 50 ) values and 50% inhibitory concentration (IC 50 ) values. The IC 50 value showed the effect of extract inhibition and was obtained from the focus assay of DENV after treated with serial concentrations of extract (80, 40, 20, 10, 5 and 2.5 μg/mL). The CC 50 value showed the effect of cytotoxic extract and resulted from MTT assay using concentrations of 640, 320 , 160, 80, 40, 20, and 10 μg /mL. The selectivity index (SI) value was ratio of CC 50 and IC 50 . The IC 50 , CC 50 and SI value of butanol fraction of Calophyllum nodosum was 5.6 μg/mL, 1181 μg/mL and 210.9, respectively.. Statistical analysis showed significant differences between control group and treatment group on focus assay and MTT assay. It can be concluded that the butanol fraction of Calophyllum nodosum had strong antiviral effect with low cytotoxic effects.

Paper Citation

in Harvard Style

Salsabila S., Calista N., Desti H. and Dewi B. (2018). The Effectivity of Butanol Fraction of Calophyllum nodosum as Antiviral Drug to Dengue Virus Serotype 2 In Vitro.In Proceedings of BROMO Conference - Volume 1: BROMO, ISBN 978-989-758-347-6, pages 124-129. DOI: 10.5220/0008358501240129

in Bibtex Style

@conference{bromo18,
author={Syifa Salsabila and Nabilla Calista and Hidayati Desti and Beti Ernawati Dewi},
title={The Effectivity of Butanol Fraction of Calophyllum nodosum as Antiviral Drug to Dengue Virus Serotype 2 In Vitro},
booktitle={Proceedings of BROMO Conference - Volume 1: BROMO,},
year={2018},
pages={124-129},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0008358501240129},
isbn={978-989-758-347-6},
}

in EndNote Style

TY - CONF

JO - Proceedings of BROMO Conference - Volume 1: BROMO,
TI - The Effectivity of Butanol Fraction of Calophyllum nodosum as Antiviral Drug to Dengue Virus Serotype 2 In Vitro
SN - 978-989-758-347-6
AU - Salsabila S.
AU - Calista N.
AU - Desti H.
AU - Dewi B.
PY - 2018
SP - 124
EP - 129
DO - 10.5220/0008358501240129