Synthesis of Glycerol –– Castor Oil Fatty Acid and Glycerol –– Oleic Acid Esters, as Emulsifier and Antibacterial Agent, Using Candida rugosa Lipase

Elmira Vania Denada, Sri Handayani, Sumi Hudiyono, Siswati Setiasih

Abstract

The research’s objective is to synthesize glycerol – castor oil fatty acid and glycerol oleic acid esters using Candida rugosa lipase in n-hexane. Molar fatty acid to glycerol ratio that used in esterification varied from 1:1, 1:2, 1:3, and 1:4. The ester products then being analysed by using FTIR. The spectra for glycerol-castor oil fatty acid ester and glycerol-oleic acid absorption peak at wave number 1735.37 cm -1 and 1748.25 cm -1 respectively, which indicate the existence of C=O ester groups. Conversion percentage in esterification was conducted using titrimetric method. The highest conversion percentage was reached at the molar ratio 1:4 with the value of 92.4% respectively for glycerol-castor oil fatty acid and 86.2% for glycerol oleic ester. Emulsifier test was performed to observe the stability and the type of emulsion, using ester product as emulsifier. The properties of the emulsifier in both esters are obtained by type of water in oil. Antimicrobial assay were also conducted for both esterification product using disc diffusion method against Propionibacterium acne and Staphylococcus epidermidis. The antimicrobial assay showed that glycerol-castor oil fatty acid esters has antimicrobial activity, but glycerol-oleic esters did not show the antimicrobial activity.

Paper Citation

in Harvard Style

Denada E., Handayani S., Hudiyono S. and Setiasih S. (2018). Synthesis of Glycerol –– Castor Oil Fatty Acid and Glycerol –– Oleic Acid Esters, as Emulsifier and Antibacterial Agent, Using Candida rugosa Lipase.In Proceedings of BROMO Conference - Volume 1: BROMO, ISBN 978-989-758-347-6, pages 62-68. DOI: 10.5220/0008357500620068

in Bibtex Style

@conference{bromo18,
author={Elmira Vania Denada and Sri Handayani and Sumi Hudiyono and Siswati Setiasih},
title={Synthesis of Glycerol –– Castor Oil Fatty Acid and Glycerol –– Oleic Acid Esters, as Emulsifier and Antibacterial Agent, Using Candida rugosa Lipase},
booktitle={Proceedings of BROMO Conference - Volume 1: BROMO,},
year={2018},
pages={62-68},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0008357500620068},
isbn={978-989-758-347-6},
}

in EndNote Style

TY - CONF

JO - Proceedings of BROMO Conference - Volume 1: BROMO,
TI - Synthesis of Glycerol –– Castor Oil Fatty Acid and Glycerol –– Oleic Acid Esters, as Emulsifier and Antibacterial Agent, Using Candida rugosa Lipase
SN - 978-989-758-347-6
AU - Denada E.
AU - Handayani S.
AU - Hudiyono S.
AU - Setiasih S.
PY - 2018
SP - 62
EP - 68
DO - 10.5220/0008357500620068