Sequence Variation of Metalloprotease Genes from Three Serratia plymuthica Isolates Collected from Rhizosphere and Pylloplant for Sustainable Agricultural Practices

Jamsari, Lily Sukraeni, Renfiyeni, Husnul Rahmi, Elly Syafriani

2018

Abstract

The effectivity of proteolitic enzyme for biopesticide is determined mainly by the nature of biological sources from which they have been isolated. Three isolates identified as Serratia plymuthica were isolated from rhizsosphere of onion and phylosphere of cabbage. In order to maximize their capability as biopesticide we isolated and characterized their metalloprotease encoding gene and looked deeper for genetic manipulation possibility. All the three strains have similar length of 1059 bp which is shorter than that previously expected 1107 bp. Seven point mutations along the gene sequences were observed. However, only 780 bp could be predicted as ORF and gave 259 amino acids sequences for each strains. Domain analysis predicted that the gene contained M48C_loiP-like motif started from 115-762 base. The domain also contains Zn binding motif (HEXXH) indicating that their activity might be influenced by the presence of Zn ion. The core ORF covering 250 amino acids contains three mutations events, causing amino acid changing on Ser-35-Phe, Ser-52-Pro and Ile-208-Asn. However, three dimentional structure and ligand binding analysis did not show any significant variation among them. The data found here, indicated that the two strains S. plymuthica share similar proteolitic capability eventhough they isolated from two different habitats

Paper Citation

in Harvard Style

Jamsari., Sukraeni L., Renfiyeni., Rahmi H. and Syafriani E. (2018). Sequence Variation of Metalloprotease Genes from Three Serratia plymuthica Isolates Collected from Rhizosphere and Pylloplant for Sustainable Agricultural Practices. In Proceedings of the International Conference on Natural Resources and Sustainable Development - Volume 1: ICNRSD, ISBN 978-989-758-543-2, pages 227-231. DOI: 10.5220/0009900500002480

in Bibtex Style

@conference{icnrsd18,
author={Jamsari and Lily Sukraeni and Renfiyeni and Husnul Rahmi and Elly Syafriani},
title={Sequence Variation of Metalloprotease Genes from Three Serratia plymuthica Isolates Collected from Rhizosphere and Pylloplant for Sustainable Agricultural Practices},
booktitle={Proceedings of the International Conference on Natural Resources and Sustainable Development - Volume 1: ICNRSD,},
year={2018},
pages={227-231},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0009900500002480},
isbn={978-989-758-543-2},
}

in EndNote Style

TY - CONF

JO - Proceedings of the International Conference on Natural Resources and Sustainable Development - Volume 1: ICNRSD,
TI - Sequence Variation of Metalloprotease Genes from Three Serratia plymuthica Isolates Collected from Rhizosphere and Pylloplant for Sustainable Agricultural Practices
SN - 978-989-758-543-2
AU - Jamsari.
AU - Sukraeni L.
AU - Renfiyeni.
AU - Rahmi H.
AU - Syafriani E.
PY - 2018
SP - 227
EP - 231
DO - 10.5220/0009900500002480