Platelet Profile in Patients with Dengue Hemoragic Fever-USU

Hospital

N. K. Arrasyid

, D. I. S. Siregar

, and K. P. W. Dasopang

Parasitology Departmen, Faculty of Medicine, Universitas Sumatera Utara,

Jl. Universitas No.1 Kampus USU Medan 20155

Clinical Pathology Department, Faculty of Pharmacy, Universitas Sumatera Utara,

Jl. dr. Mansur No. 66 Kampus USU Medan 20155

Keywords: Platelet, Dengue Hemoragic Fever.

Abstract: Medan is a DBD endemic area. There were 15 death cases in 2014 caused by DBD (Dinas Kesehatan

Provinsi Sumatera Utara, 2015). In dengue infection case, usually objects/patients will

(have/experience/suffer from) trombositopenia after they (have/experience/suffer from) fever which

excalate bleeding risk and complication. This research aims to see platelet profile on DBD patients at

Rumah Sakit Universitas Sumatera Utara. After gaining ethical clearance, blood samples are taken on the

research subject and then trombosit examinations, Mean Platelet Volume (MPV), and Platelet Distribution

Width (PDW) are performed using sysmex XT-2000i with impedance method. The gathered results are

mean trombosit of the patient is 93,9x103+-53,5x103. There are 69,6% of patients have the MPV level <9,6

fL and 65,2% of them have the PDW level ,9,2 fL.

1 INTRODUCTION

DBD is a reversible and life threatening dengue

vascular complication and related with severe

trombositopenia and increased vascular permeability

(Jayashree, Manasa, Pallavi, and Manjunath, 2011).

Trombositopenia can increase the risk of bleeding

and complications in DBD patients (Kumar, Vyas,

and Krishna, 2017). Lately, researches show that

platelet index such as MPV and PDW can be a sign

of platelet activity. The volume of platelet which is a

sign of platelet function and activity is assessed

based on MPV. Platelet activity will also cause

morphology changes from platelet into sferis form

side by side with formation of pseudopodia. In the

end, platelet with changed form and size can affect

PDW value (Navya, Patil, and Kariappa, 2016).

Medan is one of many DBD endemic areas. There

were 15 death cases in 2014 caused by DBD (Dinas

Kesehatan Provinsi Sumatera Utara, 2015).The most

prevalence of DBD case is in the group of 5-14

years old female (Departemen Kesehatan RI, 2008).

This research aims to see platelet profile on DBD

patients at Rumah Sakit Universitas Sumatera Utara.

2 MATERIAL AND METHOD

After gaining ethical clearance, samples are taken on

the research subject who has filled out the informed

concern by purposive sampling. On the selected

objects, trombosit examinations, MPV, and PDW

are performed using sysmex XT-2000i with

impedance method.

3 RESULT

This research is done on patients whos come to RS

USU from March 2018 to May 2018 and obtained

the result that shows 23 DBD patients who met the

inclusion criteria where male patients are more that

female patients with a percentege of 52,17% (table

1). This result is different from the statement of

Departemen Kesehatan RI (2008) that says the most

prevalence of DBD case is in the group of 5-14

years old female. This difference occurs might be

because men have more activity outside the house,

and vise versa.

922

Arrasyid, N., Siregar, D. and Dasopang, K.

Platelet Proﬁle in Patients with Dengue Hemoragic Fever-USU Hospital.

DOI: 10.5220/0010101509220923

In Proceedings of the International Conference of Science, Technology, Engineering, Environmental and Ramiﬁcation Researches (ICOSTEERR 2018) - Research in Industry 4.0, pages

922-923

ISBN: 978-989-758-449-7

Table 1. Characteristic of patient

Age

(Years)

Male Female

n % n %

< 18 3 13.04 2 8.70

≥ 18 9 39.13 9 39.13

Total 12 52.17 11 47.83

Trombositopenia on DBD or SSD patient might

not only be caused by direct platelet destruction by

the virus (direct cytotoxisity), but can also be caused

by platelet destruction due to binding of specific

dengue antibodies with virus infected platelet. The

other thing that might happen is that platelet can act

as dengue virus replicating place, but this issue still

needs further research. This is in line with what is

found in this study where the mean platelet of

patients is 93,9x103 +-53,5x103 uL (table 2).

Table 2. Profil Platelete of patient

o Test Laborator

Resul

1 Trombosit,

Avera

e ± sd (uL)

93.9 x10

± 53.5 x 10

2 Platelet

Distribution Width, n

(%)

 < 9.6 fL

 ≥ 9.6 fL

16 (69.5)

7 (30.4)

3 Mean Platelet

Volume, n (%)

 < 9.2 fL

 ≥ 9.2 fL

15 (65.2)

8 (34.8)

Different from the result that was obtained by

Gunawan (2010) where the PDW mean value was

9,7 fL, where in this research the result is 69,6% of

the patients are with value of PDW<9,6 fL (table 2).

However, for MPV value, this research is in line

with what Gunawan (2010) had report, that said

MPV mean value was 8,5 fL. As in this research

gained result of 65,2% of the patients have the value

of MPV <9.2 fL.

4 CONCLUSION

Trombosit mean of DBD patients in RS USU on

March – May 2018 was 93,9x103 + -53,5x103 uL.

Where 69,6% of the patients have the value of PDW

<9,6 fL and 65,2% of them have the value of PDW

<9.2% fL.

ACKNOWLEDGEMENTS

The authors gratefully acknowledge that the present

research is supported by Ministry of Research and

Technology and Higher Education Republic of

Indonesia. The support is under the research grant

TALENTA USU of Year 2018 Contract Number

2590/UN5.1.R/PPM/2018.

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Platelet Proﬁle in Patients with Dengue Hemoragic Fever-USU Hospital

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