Advancements in CRISPR Technology for Studying Antibiotic Resistance Mechanisms in Bacteria

Ziyi Xu

2025

Abstract

Antibiotic resistance (AR) is a global threat in terms of public health that lowers the efficacy of antibiotics as well as raises disease loads. While traditional genomics-based strategies have worked in detecting resistance genes in bacteria, these strategies are not reliable in functional validation of genes. The emergence of CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) technologies has ushered in a very potent gene-editing tool in understanding resistance in bacteria. This paper is a discussion on recent advancements in CRISPR-Cas9, CRISPRi, and CRISPRa application in resistance gene research, in designing antibacterial strategies, as well as in modulating metabolic pathways in bacteria. Furthermore, it explores CRISPR application in phage therapy, novel antibiotic development, as well as resistance gene modification. Future research will involve improving CRISPR delivery methods, increasing specificity in CRISPR editing, as well as incorporating synthetic biology strategies toward more potent anti-resistance therapies.

Paper Citation

in Harvard Style

Xu Z. (2025). Advancements in CRISPR Technology for Studying Antibiotic Resistance Mechanisms in Bacteria. In Proceedings of the 1st International Conference on Biomedical Engineering and Food Science - Volume 1: BEFS; ISBN 978-989-758-789-4, SciTePress, pages 124-127. DOI: 10.5220/0014431700004933

in Bibtex Style

@conference{befs25,
author={Ziyi Xu},
title={Advancements in CRISPR Technology for Studying Antibiotic Resistance Mechanisms in Bacteria},
booktitle={Proceedings of the 1st International Conference on Biomedical Engineering and Food Science - Volume 1: BEFS},
year={2025},
pages={124-127},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0014431700004933},
isbn={978-989-758-789-4},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 1st International Conference on Biomedical Engineering and Food Science - Volume 1: BEFS
TI - Advancements in CRISPR Technology for Studying Antibiotic Resistance Mechanisms in Bacteria
SN - 978-989-758-789-4
AU - Xu Z.
PY - 2025
SP - 124
EP - 127
DO - 10.5220/0014431700004933
PB - SciTePress