Chitosan Nanoparticle as a Delivery System of miRNA 217 for Suppressing Hepatocellular Carcinoma Progressivity by Targeting AEG-1/P53

Ulfatun Nisa, Indwiani Astuti, Ronny Martien, Dhani Rinaldi Maulana, Ysrafil

2020

Abstract

MicroRNA, especially miRNA-217, has important role in development of Hepatocellular Carcinoma (HCC) through its relation with astrocyte elevated gene 1 (AEG-1) who modulates several signaling arrays. Delivery systems could be crucial factors for successful gene therapy. We investigated effects of chitosan nanoparticles as delivery system of miRNA-217 for targeting AEG-1 in HCC. Chitosan nanoparticles were prepared using ionic gelation methods. Entrapment efficiency was obtained using a NanoDrop spectrophotometer. Mimic miRNA-217 encapsulated by chitosan nanoparticles were transfected in HCC cell line HepG2. Viability test was conducted by using MTT Assay. The dosages of miRNA-217 were ¼, ½ and 1 IC50. A real-time polymerase chain reaction determined miRNA-217 and mRNA relative expressions. Independent T-tests were used to analyze the parameter differences. Results showed that chitosan nanoparticles could encapsulate miR-217 with 92.9% entrapment efficiency. miR-217 was successfully delivered and significantly increase the endogenous expression of miRNA-217 in HepG2 cells compared to controls. It mediated significant cell inhibition in chitosan nanoparticles group compared to naked miRNA. The expression of mRNA AEG-1 was decreased significantly compared to controls. The increased expression of miRNA-217 was negatively correlated to AEG expression. Chitosan nanoparticles of miRNA-217 may suppress cell line progressivity via targeting AEG-1.

Paper Citation

in Harvard Style

Nisa U., Astuti I., Martien R., Maulana D. and Ysrafil. (2020). Chitosan Nanoparticle as a Delivery System of miRNA 217 for Suppressing Hepatocellular Carcinoma Progressivity by Targeting AEG-1/P53.In Proceedings of the 1st Jenderal Soedirman International Medical Conference in conjunction with the 5th Annual Scientific Meeting (Temilnas) Consortium of Biomedical Science Indonesia - Volume 1: JIMC, ISBN 978-989-758-499-2, pages 131-138. DOI: 10.5220/0010489001310138

in Bibtex Style

@conference{jimc20,
author={Ulfatun Nisa and Indwiani Astuti and Ronny Martien and Dhani Rinaldi Maulana and Ysrafil},
title={Chitosan Nanoparticle as a Delivery System of miRNA 217 for Suppressing Hepatocellular Carcinoma Progressivity by Targeting AEG-1/P53},
booktitle={Proceedings of the 1st Jenderal Soedirman International Medical Conference in conjunction with the 5th Annual Scientific Meeting (Temilnas) Consortium of Biomedical Science Indonesia - Volume 1: JIMC,},
year={2020},
pages={131-138},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0010489001310138},
isbn={978-989-758-499-2},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 1st Jenderal Soedirman International Medical Conference in conjunction with the 5th Annual Scientific Meeting (Temilnas) Consortium of Biomedical Science Indonesia - Volume 1: JIMC,
TI - Chitosan Nanoparticle as a Delivery System of miRNA 217 for Suppressing Hepatocellular Carcinoma Progressivity by Targeting AEG-1/P53
SN - 978-989-758-499-2
AU - Nisa U.
AU - Astuti I.
AU - Martien R.
AU - Maulana D.
AU - Ysrafil.
PY - 2020
SP - 131
EP - 138
DO - 10.5220/0010489001310138