Anticancer Activity of Fibraurea Tinctoria with DLD1 Celline Cytotoxicity Assay

Riski Sulistiarini, Andreanus A. Soemardji, Elfahmi, Maria Immaculata Iwo, Danang Waluyo, Dian Japany Puspitasari

2019

Abstract

This study aims to determine the cytotoxic effects of Fibraurea tinctorial plants on DLD1 colon cancer cell lines in vitro and determine the IC50 values of these plants. The test begins with cell-line culture in the DMEM medium. Cultures that were ready with an estimated concentration in fresh media of 2.5x 104cells / 200uL were exposed to 0.4uL test material in 0.1% DMSO with concentrations starting at 6.25 ppm; 12.5 ppm; 25 ppm; 50 ppm; 100 ppm; 200ppm; 400ppm; 800ppm; and 1600ppm which was then incubated for 48 hours. The results are from methanol extract, hexane fraction, ethyl acetate fraction and HA and HB subfraction from Fibraurea tinctorial plants, only HB subfaction which shows inhibitory activity against DLD1 colon cancer cell line with 28% at 1600 ppm concentration.

Paper Citation

in Harvard Style

Sulistiarini R., Soemardji A., Elfahmi., Iwo M., Waluyo D. and Puspitasari D. (2019). Anticancer Activity of Fibraurea Tinctoria with DLD1 Celline Cytotoxicity Assay.In Proceedings of the 2nd Health Science International Conference - Volume 1: HSIC, ISBN 978-989-758-462-6, pages 171-175. DOI: 10.5220/0009126401710175

in Bibtex Style

@conference{hsic19,
author={Riski Sulistiarini and Andreanus A. Soemardji and Elfahmi and Maria Immaculata Iwo and Danang Waluyo and Dian Japany Puspitasari},
title={Anticancer Activity of Fibraurea Tinctoria with DLD1 Celline Cytotoxicity Assay},
booktitle={Proceedings of the 2nd Health Science International Conference - Volume 1: HSIC,},
year={2019},
pages={171-175},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0009126401710175},
isbn={978-989-758-462-6},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 2nd Health Science International Conference - Volume 1: HSIC,
TI - Anticancer Activity of Fibraurea Tinctoria with DLD1 Celline Cytotoxicity Assay
SN - 978-989-758-462-6
AU - Sulistiarini R.
AU - Soemardji A.
AU - Elfahmi.
AU - Iwo M.
AU - Waluyo D.
AU - Puspitasari D.
PY - 2019
SP - 171
EP - 175
DO - 10.5220/0009126401710175