Isolation of DNA Genomes from the Head and Middle Gland of Silkworms (Bombyx mori L)

Masitta Tanjung, Masitta Tanjung, Saleha Hannum

2019

Abstract

The quality and quantity of DNA are very much needed for the molecular analysis of organisms. The superior quality of DNA samples can be obtained from an appropriate DNA extraction protocol.This research was conducted to isolate the DNA of silkworm larvae from the head and middle glands. The silkworms used are from three National silkworm breeding centers, namely Perhutani-Bogor, Bili-Bili and Soppeng.Organ samples taken are the head and middle silk glands. Samples are crushed and put into extraction buffers using a Mini Kit (Promega, Madison, WI, USA) in accordance with established procedures.The measurement of DNA quantity was carried out by the spectrophotometric method using a spectrophotometer at wavelengths (λ) 260 and 280 nm.DNA purity was determined by calculating the absorbance ratio at A260 to A280. DNA isolation showed a very thick band (high concentration) and also firm, this indicated that the results of DNA isolation are very good.DNA samples from the middle silk gland from the Bogor region were not obtained because the sample used when extracting was too much hence the extraction buffer used was insufficient to lyse lipids or compounds in the silkworm glands. DNA purity ranges from 1.75-2.2 with an average purity of 1.95 and the concentration of isolated DNA reaches 37,000 ng / µl.

Paper Citation

in Harvard Style

Tanjung M. and Hannum S. (2019). Isolation of DNA Genomes from the Head and Middle Gland of Silkworms (Bombyx mori L). In Proceedings of the 1st International MIPAnet Conference on Science and Mathematics - Volume 1: IMC-SciMath, ISBN 978-989-758-556-2, pages 418-421. DOI: 10.5220/0010200000002775

in Bibtex Style

@conference{imc-scimath19,
author={Masitta Tanjung and Saleha Hannum},
title={Isolation of DNA Genomes from the Head and Middle Gland of Silkworms (Bombyx mori L)},
booktitle={Proceedings of the 1st International MIPAnet Conference on Science and Mathematics - Volume 1: IMC-SciMath,},
year={2019},
pages={418-421},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0010200000002775},
isbn={978-989-758-556-2},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 1st International MIPAnet Conference on Science and Mathematics - Volume 1: IMC-SciMath,
TI - Isolation of DNA Genomes from the Head and Middle Gland of Silkworms (Bombyx mori L)
SN - 978-989-758-556-2
AU - Tanjung M.
AU - Hannum S.
PY - 2019
SP - 418
EP - 421
DO - 10.5220/0010200000002775