Isolation and Determination of Amylase Enzyme Activity from Durian Seed Sprouts

M. Zulham Efendi Sinaga, M. Zulham Efendi Sinaga, Cut Fatimah Zuhra, Cut Fatimah Zuhra, Emma Zaidar, Emma Zaidar, Firman Sebayang, Rumondang Bulan, Dea Rahmadana

2019

Abstract

One of the favorite fruits in Medan-Indonesia is durian. The number of fruit is comparable to the most waste of durian seed produced. Seeds are one of the ways to breed through the process of germination. In the process of germination which plays an important role is the enzyme amylase. Based on this, the research aims to isolate the enzyme amylase found in durian seed sprouts on the fifth day through dialysis with ammonium sulfate and then determined its activity against temperature, pH, substrate concentration. Through this research, the enzyme amylase has been isolated from the sprouts of durian beans with the best activity at temperature 40 oC (36.87 U/ml), pH 7 (36.86 U/ml), substrate concentration 1% (26.36 U/ml). The best ammonium sulfate saturation in the dialysis process is 60% acquired enzyme activity of 58.59 U/ml.

Paper Citation

in Harvard Style

Sinaga M., Zuhra C., Zaidar E., Sebayang F., Bulan R. and Rahmadana D. (2019). Isolation and Determination of Amylase Enzyme Activity from Durian Seed Sprouts. In Proceedings of the 1st International MIPAnet Conference on Science and Mathematics - Volume 1: IMC-SciMath, ISBN 978-989-758-556-2, pages 413-417. DOI: 10.5220/0010199800002775

in Bibtex Style

@conference{imc-scimath19,
author={M. Zulham Efendi Sinaga and Cut Fatimah Zuhra and Emma Zaidar and Firman Sebayang and Rumondang Bulan and Dea Rahmadana},
title={Isolation and Determination of Amylase Enzyme Activity from Durian Seed Sprouts},
booktitle={Proceedings of the 1st International MIPAnet Conference on Science and Mathematics - Volume 1: IMC-SciMath,},
year={2019},
pages={413-417},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0010199800002775},
isbn={978-989-758-556-2},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 1st International MIPAnet Conference on Science and Mathematics - Volume 1: IMC-SciMath,
TI - Isolation and Determination of Amylase Enzyme Activity from Durian Seed Sprouts
SN - 978-989-758-556-2
AU - Sinaga M.
AU - Zuhra C.
AU - Zaidar E.
AU - Sebayang F.
AU - Bulan R.
AU - Rahmadana D.
PY - 2019
SP - 413
EP - 417
DO - 10.5220/0010199800002775