Ethanol Extract (Colocasia esculenta (L.) Schott.) Hideung Cultivar

as Anti-scabies through In-Vitro

I. Gusti Ayu Manik Widhyastini

, Febi Nurilmala

and Misja

Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Nusa Bangsa, Bogor 16166, Indonesia

Keyword: Antiparasitic, Scabies

Abstract: This study aims to determine the potential of taro extracts as anti-scabies.Efforts have been made towards

the swarming number of scabies caused by the mites Sarcoptes scabiei, which attacks the skins of cattle.

Treatments relied more on synthetic chemical drugs that are more effective but are expensive. Taro plants

(Colocasia esculenta (L.) Schott) is mainly found in farmlands. Talas Bogor is a potential larvacide, able to

kill mosquito larvae, predicted to have anti-parasite properties against mites. This research includes

phytochemical tests (flavonoid, tannin, saponin, alkaloid, calcium oxalate) of the identified plant,

effectiveness test of the taro extract towards the mites through in vitro. The results of the effectiveness test

of the wild taro extract (Colocasia esculenta (L).Schott) cultivar Hideung used in in-vitro with probit

analysis obtained an LC50 value of 50.11% for 4 hours treatment time, an LC50 value of 24.54% for 6

hours’ time, able to exterminate 100% of the mites tested, using Neguvon as a positive control, and 10.96%

LC50 value for 3 hours treatment time. Treatment of wild taro extracts (Colocasia esculenta (L).Schott

Cultivar Hideung with 96% ethanol solvent is a potential alternative for scabies treatment.

1 INTRODUCTION

Scabies is generally caused by Sarcoptes

scabiei variety hominids mites, which are parasites

from the Arthropoda class with the order Acarina.

Cases of scabies infection occur in both sexes, at all

ages, groups, and all socioeconomic levels. Every

year, it is measurable that around 300 million

prevalence worldwide, this estimate might be too

high. Only female mites burrow under the host's

skin, causing intense allergic itching (Chosidow O,

2006);(Anderson and Strowd, 2017). Many of these

ectoparasite species have their breeding sites very

close to their hosts so that they are practically

always present.

Throughout the year the presence and number of

ectoparasites vary, the speed of this development

depends on the temperature, according to the season,

(especially in milder and temperate climates (Saad

and Desoky, 2016). Then stated that such activities

of prophylaxis or control need knowledge of the

developmental of the life cycle and on the periods of

occurrence of the ectoparasites as well as on their

breeding sites(Saad and Desoky, 2016). It is

necessary to develop an alternative approach in

handling ectoparasites(Fang et al., 2016) In the

rabbit; it is normally treated by injecting Ivermectin.

However, this method is expensive and is not always

effective(Vu Thi Thu Hang, Chu Duc Tuy, 2012).

Trichlorfon (Neguvon8, Bayer AG, Leverkusen)

was evaluated for its effect on naturally occurring

infestations of Sarcoptes scabiei in 16 dogs. A 0.1%

concentration of trichlorfon was applied as a wash

once a week for four weeks. Skin lesions improved

gradually and disappeared completely two weeks

after the onset of treatment. By week 4, there were

no clinical signs of scabies in any of the 16 dogs,

and no S. scabies mites or other stages of mite were

found on skin scrapings (Sarchahi, 2005). However,

the use of chemicals could result in resistance in

target species, toxicity, and environmental hazards.

(Luo et al., 2015).Based on the increase in cases of

scabies on ruminants occurring in Bogor mainly in

Babakan Madang (Misja dan Widhyastini, 2016),

actions are needed to control the increase in scabies

disease on ruminants as well as in humans. One of

the solutions to resolve this issue is by searching for

natural products that are selective, safe and

inexpensive.

Wild taro (Colocasia esculenta (L.) Schott.) If

consumed can cause itching. It is necessary to use

wild taro which is commonly found around farms

such as in fields, swamps, on the edge of nets, and

home yards. According to Fang et al., (2016) that,

natural medicines from plants have advantages such

Widhyastini, I., Nurilmala, F. and Misja, .

Ethanol Extract (Colocasia esculenta (L.) Schott.) Hideung Cultivar as Anti-scabies through In-Vitro.

DOI: 10.5220/0009940221512158

In Proceedings of the 1st International Conference on Recent Innovations (ICRI 2018), pages 2151-2158

ISBN: 978-989-758-458-9

2151

as having fewer side effects, better patient tolerance,

relatively cheaper and acceptable because of the

long history of use.

According to M Alcantara,(2013), the chemical

constituents of taro plants based on the results of

phytochemical screening have shown that taro

leaves ethanol extracts consists of compounds

including, antioxidant, phenol, flavonoids, tannins,

and saponins; and according to Krishnapriya and

Suganthi, (2017); (Kumawat et al., 2010),

preliminary phytochemical analysis of (Colocasia

esculenta (L.) Schott.) dried tubers were determined

and revealed the presence of alkaloids, glycosides,

flavonoids, terpenoids, saponins, and phenols.

In taro that is not itchy and edible that (Colocasia

esculenta (L.) Schott.), Contains a large number of

phytochemicals, so taro is recommended for the

pharmaceutical industry. Taro traditionally is used

as a medicine, the stem of the leaves is believed

to be used as the treatment for itchiness and

digestive issues in throat and asthma. The stem

can be used to heal wounds. Taro has the potential to

be an anti-scabies. According to Febi Nurilmala, et

al.,(2017), In Indonesia, the potential for taro

commodities (Colocasia esculenta (L.) Schott, which

is not itchy has not been supported by proper data

compared to other crops such as cassava, sweet

potatoes, and potatoes. Diversification of food as a

source of choice carbohydrates. Tasas are foods that

all parts of the plant, such as leaves, stems, and

tubers can be consumed. In research Adejumo,

(2013), the use of taro (Colocasia esculenta) as an

alternative energy source to feed animals. Colocasia

esculenta corm aqueous extract was assessed for it's

in vitro antioxidant capacity and free radical

scavenging potential,(Watal, Unit and Division,

2017)

2 MATERIALS AND METHODS

The materials used in this research include: wild taro

rod and leaf (Colocasia esculenta (L.) Schott.

Kultivar Hideung result of the determination of

Biological Research Center LIPI Bogor, mites

S.scabiei, ethanol 96%, filter paper, distilled water,

Neguvon. The object of the study used were rabbits

infected with scabies.

2.1 Preparation of Plant Extract

Wild taro leaf stalks were sorted and then washed,

then drained and weighed. Afterward, the

ingredients are chopped into smaller pieces, dried by

air for 2x24 hours. After drying, it was then put into

the oven for three days at 60 ° C so that the sample

is arid and then weighed dry and blended until it

becomes smooth to form a powder.

The extract of the wild taro extract was carried

out by maceration method, which is the material that

has become powder and then taken as much as 200

grams of powder, then macerated with 96% ethanol

as much as 1000 ml for 72 hours. Followed by

filtration to produce a filtrate. The filtrate is

evaporated using a rotary evaporator, then

evaporated above the water bath until the volume

becomes 10% of the initial volume and until a thick

extract is obtained.

2.2 Phytochemical Screening

The phytochemical screening method based on

(Dhanraj et al., 2013) are as follows:

 Detection of Alkaloids

The extract is dissolved individually in a

hydrochloric acid solution and then filtered.

The filtrate is treated with Hager reagent

(saturated picric acid solution). The formation

of yellow deposits indicates the presence of

alkaloid compounds;

 Detection of Tannin

The 4 ml extract was treated with 4 ml FeCl,

after which appears a green color, concluding

as positive for tannin;

 Detection of Flavonoids

Alkaline reagent test; the extract is dripped

with a few drops of 10% sodium hydroxide

solution. Intense yellow formation becomes

colorless on the addition of dilute acid,

indicating the presence of flavonoids ;

 Detection of Saponin

The extract was diluted with distilled water up

to 20 ml, and this was shaken in a graduated

cylinder for 15 minutes. Formation of 1 cm

layer foam indicates the presence of saponin.

The 0,5 g of extract was shaken with 2 ml of

water. If foam persists to produce for ten

minutes, it indicates the presence of saponins;

 Measurement oxalate levels

Taro powder sample weighed approximately

0.5 grams in Erlenmeyer grindstones and

dissolved in water as much as 38 ml and 1 ml

of 6N hydrochloric acid. The solution was then

heated at 100°C, cooled and filtered. The

filtrate was divided into two parts; each section

is titrated with ammonium hydroxide using

methyl red indicator until the red color changed

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to yellow. The results of titration were heated

at 90°C with constant stirring, then add 2 ml of

5% calcium chloride and cooled by

maintaining a constant stirring, allow the cool

solution to stand overnight. The next stage is

the separation of the precipitate, and the

supernatant is done using centrifuges with a

speed of 2.500 rpm for 5 minutes. The

precipitate is dissolved in sulfuric acid 20%.

All solutions are combined and made into 40

ml. 25 ml of solution titrated with potassium

permanganate solution 0,05N with an end point

indicated by the formation of a pink color that

persists for 30 seconds.

2.3 Mite Sample Preparation

The mite samples used in this study were Sarcoptes

scabiei mites obtained from rabbit skin scrapings

infested with S. scabiei naturally (Luo et al., 2015);

(Sashidharan, Basavaraj, and Bates, 2016);(Ari

Puspita Dewi .Haryuningtyas, 2008). The infested

skin was scraped using a scalpel or cutter knife until

it bled, then the scrapings were collected into a petri

dish (Sashidharan, Basavaraj, and Bates, 2016);(Ari

Puspita Dewi .Haryuningtyas, 2008) which was

filled with physiological NaCl. Each petri dish was

filled with ten mite samples (Ari Puspita Dewi

.Haryuningtyas, 2008).

2.4 Test the Effectiveness of Wild Taro

Extract

The method of test the effectiveness of wild taro

extract, are as follow:

 Preliminary test

The preliminary test begins with the

concentration of the concentrated extracts into

the following concentrations of 0%, 20%, 40%,

60%, and 80%. Each treatment consisted of 10

Sarcoptes scabiei with three replications, to

determine the concentration of which treatment

is causing death by 50% of the population of

Sarcoptes. Criteria for the death of Sarcoptes is

that they do not move or do not react when

touched in the treatment. A positive control

using Neguvon and for negative control using

distilled water. The treatment stood for 6 hours;

then observation was done every hour under a

microscope with 100x magnification, the data

of dead mite was recorded and analyzed;

 Effectiveness Test

In the effectiveness test, the concentration used

was the concentration obtained in the

preliminary test which caused 50% death of the

Sarcoptes population, which became the

reference for determining the range of

concentration intervals on the actual toxicity

test, i.e., by raising and lowering the

concentration in the preliminary test, by a

close-range interval. The test concentration

used were: 0, 25%, 30%, 35%, 40%, 45% and

50%. Treatment of Neguvon used were 10%,

20%, and 25%. Calculation of dead mites is

carried out every hour; observations carried out

for 6 hours. The data obtained were then

analyzed using probit analysis to determine the

LC50 value in each treatment hour;

 Data Analysis

The data obtained were then analyzed using a

Probit analysis to determine the LC50 value in

each hour of treatment.

This analysis is used in biological testing to

determine the response of the subjects studied by the

presence of stimuli, in this case of wild taro extract

by knowing the response in the form of mortality of

mites.

3 RESULTS AND DISCUSSION

Based on the "Herbarium Bogoriense," LIPI Bogor

Botanical Field of Biological Research Center, wild

taro is used in taro type of Colocasia esculenta (L)

Schott. Hideung Cultivar.

3.1 Secondary Metabolism

The assessment of a plant can be considered a food

source, the main thing to note is the nutritional value

of a plant. However, the presence of other toxic

compounds in a plant can also affect plant

characteristics and nutritional content. These toxic

factors act as anti-nutrients and affect organisms.

Antinutrients are chemicals which have been

evolved by plants for their defense, among other

biological functions. Anti-nutrients reduce the

maximum utilization of nutrients(especially proteins,

vitamins and minerals), thus preventing optimal

exploitation of the nutrients present in food and

decreasing the nutritive value (Mcewan, 2008).

Anti-nutrients vary in chemical structures, ranging

from amino acids to proteins; from simple amines to

a1kaloids, glycosides and many phenolic

compounds. The biological effects of all these

Ethanol Extract (Colocasia esculenta (L.) Schott.) Hideung Cultivar as Anti-scabies through In-Vitro

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chemicals are diverse and complex. Analysis of

secondary metabolites was carried out using extracts

from Bogor taro leaves and stems (Colocasia

esculenta (L) Schott) Hideung Cultivar identifies

compounds such as alkaloid, flavonoid, tannin,

saponin, and calcium oxalate compounds, (Table 1).

The anti-nutrient levels were generally low and thus

may not pose an immediate effect on the health of

consumers. Reduction of the antinutrients through

processing (cooking, frying, roasting) was observed

to enhance the nutritional value of these tubers.

(Mcewan, 2008).

The results of the phytochemical

test showed that there were flavonoids, tannins,

saponins, and calcium oxalate. According to

Temesgen and Ratta,( 2015),anti-nutrients which

found in taro root have negative implications for taro

as food, yet they also have positive implications for

taro as a crop that can be grown with minimal use of

fungicides and pesticides. The main antinutrients

that exist in taro are mucilage, oxalic acid, tannins,

cyanide, lectins, alpha-amylase inhibitors, protease

(trypsin and chymotrypsin) and

inhibitors)(Temesgen and Ratta, 2015).

Flavonoids function as anti-bacterial by forming

complex compounds against extracellular proteins

that interfere with the integrity of bacterial cell

membranes (Kumar and Pandey, 2013). In addition

to flavonoids which have the ability as an anti-

bacterial, alkaloids can also be an anti-bacterial.

(Kumar and Pandey, 2013); (Cushnie, Cushnie, and

Lamb, 2014) The suggested mechanism is by

disrupting the constituent components of

peptidoglycan in the cell so that the cell wall layer is

not formed completely and causes the cell death

(Cushnie, Cushnie, and Lamb, 2014). In the test

results of leaf extracts and stems of wild taro plants,

showed the presence of tannin compounds.

According to Ashok and Upadhyaya, (2012) medical

tannins can be used, among others, to heal burns,

stop bleeding, stop the infection, heal wounds

internally antidiarrheal, hemostatic, and ant

hemorrhoids. Tanin can form a protective layer over

the open tissue to make the wound uninfected even

more.

Saponins are secondary metabolites with high

molecular weight. They present in a wide range of

plant species and are distributed throughout the bark,

leaves, stems, roots and even flowers (Moghimipour

and Handali, 2015). Saponins are bitter in taste

Saponin has the ability as an anti-septic which

functions to kill germs (Nimenibo-uadia, Ugwu, and

Erameh, 2017), hepatoprotective, anti-ulcer, anti-

tumor, antimicrobial, adjuvant and anti-

inflammatory activities (Moghimipour and Handali,

2015).

Oxalate compound is generally known anti-

nutrients found in taro, which creates a feeling sharp

stabbing. Oxalate is also in the know can form

complexes with metal compounds that reduce

nutrient bioavailability of minerals in taro.

Compounds are known calcium oxalate is insoluble

in water and has a tendency to precipitate

(solidified) in the kidneys or the urinary tract, thus

forming calcium oxalate crystals acute when the

concentration is high enough (M Alcantara, 2013).

Reported that oxalate levels were higher in petioles

of taro cocoyam than in leaves (du Hang and

Preston, 2010). Oxalate salts, such as sodium and

potassium, can dissolve, whereas calcium oxalate

salts are insoluble. Calcium oxalate is a salt formed

from oxalic acid. Calcium oxalate tends to settle in

the kidneys or in the urinary tract to form calcium

oxalate crystals; these crystals play a role in the

formation of kidney stones at high levels, sharp-edge

calcium oxalate crystals, (Mcewan, 2008);(Watal,

Unit and Division, 2017). Colocasia esculenta

has been broadly investigated for proximate

composition and antinutrient screening, but the data

were not comparable because of variations in

genotypes, locations, and experimental analysis.

Generally, taro plants, have an astringent and itchy

taste that can cause swelling of the lips, mouth, and

throat if eaten without proper processing, (Mcewan,

2008). Oxalate compounds found in taro plants are

chemical compounds responsible for poisoning,

oxalate compounds are also found in other plants, (E

Yuen, 2001).

Table 1: Qualitative analysis of some metabolites of

extract Colocasia esculenta (L) Schott. Hideung Cultivar

in alcohol extract of leaf and stem

Sample

Flav

onoi

Tani

Saponi

Alk

aloi

Ca-

Oksal

Leaf

1 ++ ++ + - +

2 ++ ++ + - +

3 ++ ++ + - +

Stem

1 ++ ++ + - +

2 ++ ++ + - +

3 ++ ++ + - +

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3.2 The Potential Test of Wild Taro

Extract

The potential test of wild taro extract was carried out

by a bioassay test method by calculating the probit

value to determine LC50. The test was performed in

two experimental stages: preliminary test and

effectiveness test of extract. Collection of scraped

mite samples from rabbits infected with scabies is

collected in a petri dish. The scraping is done at

room temperature at about 22-30 ° C. This is to

ensure samples survive during testing and as a

requirement in testing the potential of the extract.

According to (Chosidow, O. 2006) Sarcoptes

scabies mites have a temperature of less than 27-30

°C which is approximately four days outside the host

and still caton infect and penetrate. Female mites

live longer than male mites in the same condition.

3.2.1 Preliminary Test

The preliminary test used variations of extract stalks

and leaves of wild taro (Colocasia esculenta (L.))

Schott hideung cultivar. This is the effect of the

addition of wild taro extract concentration by

determining the LC50 of mortality. The

concentrations used were 0%, 20%, 40%, 60%, and

80%. The calculated probitical value of the initial

test of wild taro extract for 6 hours is 28.18%.

y=3,4532x‐ 0,0165

R²=0,9958

‐2

00,511,52

probitofmortality

log10concentration



Figure 1: Calculating LC50 using probit analysis on

equations for 6 hours in preliminary test

LC50 value of the toxicity of wild taro extract

against Sarcoptes scabies mites in preliminary tests

every hour at initial concentrations can be seen in

Table 2.

Table 2: Result of LC50 Ethanol Extract of Wild Taro in

the Preliminary Test

Ethanol Extract of Wild Taro

Time(hour) LC

(%)

1 169,8

2 109,6

3 69.1

4 53.7

5 45.7

6 28.18

The LC50 value of the toxicity of wild taro

extract on Sarcoptes scabies mites on the

effectiveness test every hour at the actual

concentration can be seen in Table 2. LC50 values

obtained at the preliminary test concentration (0%,

20%, 40%, 60%, and 80%) in every hour. Based on

the results, the LC50 value of 28.18% is the smallest

concentration of extracts that can kill 50% of test

mites for 6 hours. The LC50 value will be used as

the concentration range in the actual test.

3.2.2 Test the Effectiveness of Wild Taro

Extract

The concentration toxicity test used in the actual

stage refers to the concentration of the preliminary

test (LC50) which is in the concentration range of

28.18% (table.1), so that the actual test used

concentration of 0; 25; 30; 35; 40; 45; and 50%, the

test was carried out with three repetitions, and

Neguvon was tested as a positive control.

The results of the acquisition of mite’s mortality

percentage data showed that the percentage increase

increased in each increase in the concentration of

treatment. Acquisition of mortality percentage on the

effectiveness test of wild taro extract was able to kill

mites up to 100% with a concentration of 50%

extract for 6 hours.

0204060

Mitesmortality

(%)

Extractconsentrations

ho…



Figure 2: Probit analysis of mites mortality with extract

concentrations for 1-3 hours

Ethanol Extract (Colocasia esculenta (L.) Schott.) Hideung Cultivar as Anti-scabies through In-Vitro

2155

100

120

0 204060

Mitesmortality(%)

Extractconsentrations

hou



Figure 3: Probit analysis of mites mortality with extract

concentrations for 4-6 hours

The data obtained from the percentage of mite

mortality showed at the first hour at 25% extract

concentration was able to kill 6% of the test mites,

30% extract concentration was able to kill 10% of

the test mites, while the extract concentration of

35% was able to kill mites by 13% of the test mites.

Then at a concentration of 40% increased to 17%, at

the concentration of 45% was able to kill mites 23%

and a maximum at 50% extract concentration at the

first hour of mite mortality percentage of 27%

(Figure 2).

The percentage of mortality increased along with

the increase in concentration at every hour, up to the

6th hour at 50% extract concentration capable of

killing 100% mites from test mites (Figure 3).

If calculated based on the calculation of probit

analysis from observational data every hour of

treatment, it requires different extract concentrations

to kill 50% of the test mite population — the results

of the calculation of the probit value obtained by the

linear regression equation with the predicted LC50

value of 24.54% for 6 hours.

Table 3: A Comparison of LC50 for Extracts of Wild

Taro Hideung Cultivar and Neguvon

Time

(hour)

Ethanol Extract

of Taro

(%)

Neguvon

(%)

1 93.3 87.09

2 70.79 42.65

3 64.56 10.96

4 50.11

5 39.80

6 24.54

At 2 hours, it was predicted that the LC50 value

for wild taro extract was 70.79%, meaning that at 2

hours effective concentration treatment to kill 50%

of test mites required extract concentrations is of

70.79%.

Then the LC50 value at 3 hours obtained

64.56%, at 4 hours obtained the LC50 value of

50.11%, while at 5 hours obtained the LC50 value of

39.80%. The lowest extract concentration obtained

at six hours is 24.54% (table3).

The positive results of the LC50 values obtained

from wild taro extract with 6 hours of observation

were 24.54%, at that concentration wild taro extract

was able to kill mites up to 100%. The results of

LC50 values on Neguvon as a positive control, at 3

hours of treatment were able to kill mites up to 50%

at a concentration of 10.96%, (Table 3).

Recent studies suggested that aqueous and

ethanol extracts from plants used in allopathic

medicine are potential sources of anti-viral, anti-

tumor and anti-microbial agents, etc. (Prajapati et

al., 2011). According to (Sarchahi, 2005)

Trichlorfon (metrifonate) is an organophosphate

used as an insecticide and pesticide for plants and

livestock (Neguvon) as well as an anthelmintic for

animals, principally the horse. It can be used

systemically in dogs for its anthelmintic pesticidal

properties. Thus, it can be recommended that a 0.1%

solution of trichlorfon is an effective and safe

treatment for canine sarcoptic mange, but beyond

the limitations of the study, the results of research on

wild taro extract still have the potential as an

acaricide because it can kill 50% mites within 6

hours with a concentration of 24.54%. This shows

that the extract of wild taro has the potential as an

anti-scabies.

According to Hay et al., (2012), the pathogenesis of

scabies involves many complex immunological and

inflammatory pathways, some of which we have just

begun to understand. Inflammation of the skin,

papules, and pruritus results from a hypersensitivity

reaction delayed by specific immune-mediated

antigens. Beginning 3-4 weeks after the main

infestation is usually asymptomatic. In subsequent

infestations, however, symptoms reappear much

more quickly, in about 1-2 days. Further action

aimed at achieving realistic control targets must

involve further research topics, such as long-term

health consequences of scabies infection or an

explanation for cycle events, and purification and

trials of appropriate regimens for community-based

scabies control using ivermectin and topical agents

and options for the treatment of scabies, (Luo et al.,

2015). All different attacks of part-time ectoparasites

and endoparasitic lice, mites, or insects make it

necessary to block their aggressive attacks with

ICRI 2018 - International Conference Recent Innovation

2156

repellents to be placed on animals and to reduce the

number of aggressors around the herd with

insecticide applications. And acaricides on the skin

of farm animals and the ectoparasite breeding sites.

Such activities of prophylaxis or control need

knowledge of the developmental of the life cycle

and on the periods of occurrence of the ectoparasites

as well as on their breeding sites (Saad and Desoky,

2016).

However, the use of chemicals could result in

resistance in target species, toxicity, and

environmental hazards, Natural medicines from

these plants are gaining popularity because of

several advantages such as often having fewer side

effects, better patient tolerance, relatively cheaper

and acceptable because of the long history of use

(Tabassum and Hamdani, 2014)

4 CONCLUSIONS

A plant will be a food source, especially getting

attention is its nutritional content. However, the

content of other compounds that are toxic to a plant

will affect the nutritional value of the plant.

Analysis of secondary metabolites was carried out

using extracts from Bogor taro leaves and stems

(Colocasia esculenta (L) Schott) Hideung Cultivar

identifies compounds such as alkaloid, flavonoid,

tannin, saponin, and calcium oxalate compounds.

Colocasia esculenta has been broadly investigated

for proximate composition and antinutrient

screening, but the data were not comparable because

of variations in genotypes, locations, and

experimental analysis.

 Most taro cultivars have an

astringent taste and can cause swelling of lips,

mouth, and throat if eaten unprocessed.

Implications for taro as food, yet they also have

positive implications for taro as a crop that can be

grown with minimal use of fungicides and

pesticides. Collection of scraped mite samples from

rabbits infected with scabies is collected in a petri

dish. The scraping is done at room temperature at

about 22-30 ° C. This is to ensure samples survive

during testing and as a requirement in testing the

potential of the extract. Wild taro extract (Colocasia

esculenta (L). Schott) Hideung Cultivar with 96%

ethanol solvent has potential as anti-scabies with the

LC50 value of 24.54% capable of killing 100%

mites within 6 hours. Neguvon is effective for

sarcoptic, but beyond the limitations of the study,

the results of research on wild taro extract still have

the potential as an acaricide. This shows that the

extract of wild taro has the potential as an anti-

scabies. However, the use of chemicals could result

in resistance in target species, toxicity, and

environmental hazards

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Adejumo, I. (2013) ‘Colocasia esculenta (L.) Schott as an

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Office,' The Journal of the American Board of Family

Medicine, 30(1), pp. 78–84. doi:

10.3122/jabfm.2017.01.160190.

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