2 MATERIALS AND METHODS 
A field trial was set up in the Immature Plants Palm 
Oil Plantation, PT. FajarAgung Lestari North 
Sumatera, PerbaunganSerdangBedagai. The study 
was conducted on 6 April to 10 June 2017. 
Experimental plots with each size of 3 x 3 m
2 
were 
laid in a randomized complete block design with 3 
replications. The summed dominance ratio ofA. 
gangetica and Ottochloanodosa were 32 and 37%, 
respectively. The other weeds were 
Puerariajavanica(15%) and mixed of Cynodonsp, 
Colocasiasp, Centrocemasp (14%).  
The treatments were Glyphosate-isopropyl 
ammonium 1.5 l/ha + Metsulfuron-methyl 150 g/ha; 
Paraquat dichloride 2 l/ha; 2,4-D Dimethyl amine 
2.5l/ha and untreated control (water). Spray volume 
was 450 l/ha using conventional Solo Knapsack 
Sprayer with red flat-fan nozzle of 5/64 inch orifice 
diameter, 1-1.5 bars pressure. Assessments were 
done on percent yellowing of treated weeds at 3 days 
after treatment (DAT) and percentage of weeds 
killed was carried out at 7, 14, 21, 28 DAT. Re-
growth of weed was evaluated at 30, 44 and 58 
DAT. Wet and dry weight of weed were also 
evaluated at 58 DAT (from square quadrate of 0.5 
x0.5m). 
One-way ANOVA was done to determine the 
effect of treatments on the weed. If ANOVA results 
were significant, LSD Test (P = 0.05) were used to 
separate the means. Data of weed yellowing, weed 
control and re-growth was normalized 
5.0x
(Gomez & Gomez, 1984) before analysis. All 
statistical analyses were run on the MINITAB 
Statistical Package (Minitab vol.16, 2010). 
3 RESULTS AND DISCUSSIONS 
Herbicides affected weed yellowing. Both A. 
gangetica and O. nodosa showed high weed 
yellowing (up to ±80%) caused by Paraquat 
dichloride 2 l/ha, whilst 2,4-D Dimethyl amine 
2.5l/ha caused of 57.33 and 76.66%, respectively. 
Application of Glyphosate-isopropyl ammonium 1.5 
l/ha + Metsulfuron-methyl 150 g/ha caused lowest 
A. gangetica and O. nodosa yellowing (23.33 and 
43.33%) (Table 1). 
The high effect of Paraquat dichloride 2 l / ha on 
A. gangetica and O. nodosa showed yellowing of 
leaves, and some of them were brown, even black , 
especially leaves at weed canopy. However, the 
leaves, stems and branches on the bottom position 
were still alive. Paraquat dichloride is a contact 
herbicide acting by inhibition of respiration and 
photosynthesis, destroying the plants foliar system 
(Sebayang, 2005). 
Application of  2,4-D Dimethyl amine 2.5 l / ha 
caused 100% of A. gangeticakilled at 21 DAT. It 
might causea selective herbicides, poisonous 
especially to broadleaf weeds, mainly absorbed by 
leaf and also through the soil. Whilst, Glyphosate-
isopropyl ammonium 1.5 l/ha + metsulfuron-methyl 
150 g/ha caused lowest A. gangeticakilled at 7 DAT, 
and it increased consistently up to 100% at 28 DAT. 
Glyphosate is a systemic herbicide and it is much 
more effective against weed with translocated 
readily through the plant.  Paraquat dichloride 
generally seems to be similar effective with 2,4-D 
Dimethyl amine 2.5 l/ha in controlling A. gangetica.  
Paraquat dichloride 2l/ha caused high effect onO. 
nodosakilled (up to 80%) at 7 DAT, whilst 
Glyphosate-isopropyl ammonium 1.5 l/ha + 
metsulfuron-methyl 150 g/ha and 2,4-D Dimethyl 
amine 2.5 l/ha only caused weed killed 13.33 and 
33.33%, respectively. Application of 2,4-D 
Dimethyl amine showed weed killed ranging 30 to 
40% until 28 DAT, but Glyphosate-isopropyl 
ammonium 1.5 l/ha + metsulfuron-methyl 150 g/ha 
showed weed killed until 28 DAT and it caused 
68.33% of weed killed. Generally, herbicides of 2,4-
D Dimethyl amine 2 l/ha showed less effective in 
controlling  O. nodosa (Table 1). According to 
Nasution (1986
a
)  2.4-D compounds included in 
selective herbicides, are toxic especially to broadleaf 
plants. The selectivity properties of 2,4-D herbicides 
occur on the basis of recovery in broad-leaved plants 
greater than that in tanning plants due to interception 
and higher spray solution retention. 
Paraquatdichloride 2 l/ha showed high effect at 
early evaluation, but it decreased the effect at 14, 21, 
and 28 DAT. Control of O. nodosa with a paraquat 
herbicide did not show effective results because the 
stolen part of weeds was not exposed to herbicides. 
Consequently, new leaves continued to grow in the 
next period.  According to Wibawa et al. (2009), 
some annual grasses may only be temporarily 
suppressed by paraquaat, because the low and 
enclosed growing points are not contacted by the 
spray.  
A 38.33% A. gangetica re-growth was observed 
in the Paraquat dichloride 2 l/ha application.  
Observation at 58 DAT also indicated the faster re-
growth duration than other application due to their 
relatively higher dry weights at 58 DAT (Table 2). 
Application 2,4-D Dimethyl amine on A. 
gangetica(Table 2)  showed the lowest weed re-