Comparison of Mean Platelet Volume in Urticaria and Non Urticaria

Patient in Dr. Saiful Anwar Regional Hospital Malang

Doni Kristanto, Aunur Rofiq and Dhelya Widsmara

Department of Dermatology and Venereology, Faculty of Medicine, Brawijaya University, Dr Saiful Anwar Regional

Hospital Malang., Indonesia

Keywords: Mean Platelet Volume, Urticaria, Non-urticaria.

Abstract: Increased of Mean Platelet Volume (MPV) is one of potential marker for disease activity especially for

urticaria based on extrinsic coagulation pathway activity, alternative complement pathway and autoimmune.

Another study showed decreased on MPV in chronic urticaria. This study is aimed to compared MPV value

in urticaria and non-urticaria patient. This analytic observational cross-sectional study was conducted with

consecutive sampling and has been held in June – August 2017. The term of inclusion and exclusion criteria

was applied. Data was analyzed using independent T test. Anamnesis, physical examination dan blood

sampling were done in 23 urticaria and non-urticaria patients each. Data showed increased MPV serum in

urticaria compared with non-urticaria patients but this difference was non-significant (sig 0,393; p>0,05). The

conclusion is non-significant difference statistically found in serum MPV value between urticaria and non-

urticaria patient, the average MPV value in urticaria patient was higher that non urticaria patient. Urticaria

type were thought to have a role in MPV value.

1 INTRODUCTION

Mean Platelet Volume (MPV) is simple and accurate

marker to evaluate platelet function and activity. It’s

regularly used in complete blood count examination

that point to platelet volume

(Dugyu, 2015). Platelet

size and volume were depended on bone marrow

production. MPV wasn’t correlated with platelet ages

in circulation

(Giuseppe, 2009). Investigation

showed that MPV can be used as inflammation

marker. Acute urticaria often accompanied with

inflammation like fever and leukocytosis

(Dugyu,

2015), (Vagdatli, 2010).While in chronic urticaria

found strong evidence of increased MPV value

compared to healthy subject

(Kolkhir, 2016). Cohen

et al (2012) found that high MPV value was the most

commonly marker found in chronic urticaria (3552

from 12778 patients)

(Cohen, 2012).But some

investigator found decreased in MPV value in chronic

urticaria, so it is still controversial

(Isiksacan, 2014).

Based on that difference, the author what to

investigate the comparation of MPV value in urticaria

and non urticaria patient in di Dr Saiful Anwar

Regional Hospital Malang.

2 METHODS AND RESULTS

This study used cross-sectional observational analytic

design in Dermatology and Venereology Outpatient

Clinic and Clinical Pathology Laboratorium In Dr

Saiful Anwar Regional Hospital Malang, East Java,

Indonesia. The study was approved by the Hospital

Ethics Committee then carried out from June to

August 2017.

2.1 Patients and Clinical Evaluation

The study sample was 23 urticaria patient and 23 non

urticaria patient in Dermatology and Venereology

Outpatient Clinic Dr Saiful Anwar Regional Hospital

Malang. Inclusion criteria were urticaria and non

urticaria patients, aged 18-65 years old, agreed to sign

the informed consent. Exclusion criteria were no

physical urticaria and vasculitis history, obesity,

pregnancy, hypertension, diabetes, active smoker,

skin inflammation, vitiligo, dental infection, sinusitis,

upper respiratory and urinary tract infection, and

aspirin medication in the last 3 month.

Kristanto, D., Roﬁq, A. and Widasmara, D.

Comparison of Mean Platelet Volume in Urticaria and Non Urticaria Patient in Dr. Saiful Anwar Regional Hospital Malang.

DOI: 10.5220/0008150000280030

In Proceedings of the 23rd Regional Conference of Dermatology (RCD 2018), pages 28-30

ISBN: 978-989-758-494-7

2.2 Mean Platelet Volume (Mpv) Value

Measurement

Blood sample was taken as much as 5 ml in EDTA

vacutainer tube and processed immediately using

Hematology Analyzer Sysmex XN 1000

automatically to get MPV value in Clinical Pathology

Laboratorium In Dr Saiful Anwar Regional Hospital

Malang

2.3 Data Analysis

Data entry was done on the data sheet. The data then

processed with computer program Statistical Package

for Social Sciences (SPSS). Normality test using

Shapiro-Wilk test and homogenity using Levene test.

The comparison of MPV value in urticaria and non

urticaria was analysed using independent T test.

There were 23 urticaria and non urticaria patients

each aged 18-62 years old included in this study as

showed in table 1 baseline characteristic. Average

MPV value in urticaria group was 9,72 fL ± 0,71. The

lowest MPV value was 8,00 fL and the highest MPV

value was 11,2 fL. Average MPV value in non

urticaria group was 9,56 fL ± 0,58. The lowest MPV

value was 8,20 fL and the highest MPV value was

10,5 fL. Normality test using Shapiro-Wilk test

showed that both group have normal distribution.

Homogenity test using Levene test showed that data

was homogen. Independent T Test was used to

compare MPV value in urticaria and non urticaria

group. From the table 2 showed that t

count

was 0,863

with significancy 0,393. Non-significant difference in

MPV value between urticaria group compare with

non urticaria group can be concluded from t

count

< t

table

or significant value is bigger than α 5%, where the

average MPV value urticaria group is higher than non

urticaria group.

Table 1: Baseline Characteristic.

Table 2: Independent Sample t.

3 DISCUSSION

Average MPV value was higher in urticaria compared

with non urticaria showed there is platelet mediator

roles in urticaria pathogenesis or stimulation on bone

marrow secondarily with increased bigger platelet

production as result that caused by increased platelet

consumption on urticaria wheal or systemic

inflammation

(Milovanovic, 2004), (Canpolat, 2010),

(Purnak, 2011). Platelet role in extrinsic coagulation

pathway lead to histamine release and affect disease

activity

(Aleem, 2015). Bigger platelets were more

reactive than normal platelets, with higher

tromboxane B2 production (Giuseppe, 2009),

(Martin, 1983). MPV was suitable for long time

Comparison of Mean Platelet Volume in Urticaria and Non Urticaria Patient in Dr. Saiful Anwar Regional Hospital Malang

obsevation of inflammation reaction because it was

not correlate with other measurement

(Milovanovic,

2004). Non-significant difference caused by urticaria

type was not considered. According to Vena GA et al

(Vena, 2016), platelet activation is more common in

chronic urticaria

(Vena, 2016). While in acute

urticaria, direct interaction between alergen and IgE

spesific antigen on mast cell and basophil and trigger

mast cell degranulation

(Allen, 2012).

4 CONCLUSIONS

The average MPV value in urticaria patient is higher

than non urticaria patient, but it has non-significant

difference statistically. This may caused by all

urticaria type were included without selected in to

acute or chronic urticaria as platelet activation

process more often in chronic urticaria.

ACKNOWLEDGEMENT

This study was funded by Ministry of Health

Republic of Indonesia since the author was joining

Scholarship from this institution.

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