Phenotypic and Molecular Detection of OXA-48 Gene Carbapenem-resistant Klebsiella Pneumoniae and Escherichia Coli Isolates in Haji Adam Malik Hospital Medan, Indonesia

Mirzan Hasibuan, R. Lia Kusumawati, Dwi Suryanto

2018

Abstract

Carbapenem-resistant to Klebsiella pneumoniae and Escherichia coli, increasingly reported as a major cause of infection in hospitals and healthcare facilities. Carbapenemase is an enzyme produced by gram-negative bacteria that causes failure of antibiotic therapy, especially the carbapenem. The study aimed to characterize phenotypically using Vitek 2 Compact and detect OXA-48 clinical isolates of Klebsiella pneumoniae and Escherichia coli belonging to Carbapenem-Resistant using PCR. The results showed that all were betalactamase producers, which of 12 (14.11%) were resistant to carbapenem. The phenotype distribution of carbapenem is 10/12 (11.75%) Klebsiella pneumoniae and 2/12 (2.35%) Escherichia coli. From 12 isolates Carbapenemase phenotypically, in which 10 (11.77%) of both bacteria bearing OXA-48 gene with the distribution 9 (10.6%) Klebsiella pneumoniae and 1 (1.17%) Escherichia coli, respectively. The study shows that prevalence of OXA-48 genes in North Sumatra, Indonesia in Klebsiella pneumoniae and Escherichia coli which cause failure of therapeutic types of antibiotics Carbapenem. The only antibiotic that is still sensitive to carbapenem-resistant bacteria based on antimicrobial susceptibility is amikacin, which can be recommended as carbapenemases therapy.

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Paper Citation


in Harvard Style

Hasibuan M., Kusumawati R. and Suryanto D. (2018). Phenotypic and Molecular Detection of OXA-48 Gene Carbapenem-resistant Klebsiella Pneumoniae and Escherichia Coli Isolates in Haji Adam Malik Hospital Medan, Indonesia.In Proceedings of the International Conference of Science, Technology, Engineering, Environmental and Ramification Researches - Volume 1: ICOSTEERR, ISBN 978-989-758-449-7, pages 590-594. DOI: 10.5220/0010079805900594


in Bibtex Style

@conference{icosteerr18,
author={Mirzan Hasibuan and R. Lia Kusumawati and Dwi Suryanto},
title={Phenotypic and Molecular Detection of OXA-48 Gene Carbapenem-resistant Klebsiella Pneumoniae and Escherichia Coli Isolates in Haji Adam Malik Hospital Medan, Indonesia},
booktitle={Proceedings of the International Conference of Science, Technology, Engineering, Environmental and Ramification Researches - Volume 1: ICOSTEERR,},
year={2018},
pages={590-594},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0010079805900594},
isbn={978-989-758-449-7},
}


in EndNote Style

TY - CONF

JO - Proceedings of the International Conference of Science, Technology, Engineering, Environmental and Ramification Researches - Volume 1: ICOSTEERR,
TI - Phenotypic and Molecular Detection of OXA-48 Gene Carbapenem-resistant Klebsiella Pneumoniae and Escherichia Coli Isolates in Haji Adam Malik Hospital Medan, Indonesia
SN - 978-989-758-449-7
AU - Hasibuan M.
AU - Kusumawati R.
AU - Suryanto D.
PY - 2018
SP - 590
EP - 594
DO - 10.5220/0010079805900594