Thomas Windbacher, Viktor Sverdlov, Siegfried Selberherr, Clemens Heitzinger, Norbert Mauser, Christian Ringhofer



In this work the properties of a biotin-streptavidin BioFET have been studied numerically with homogenized boundary interface conditions as the link between the oxide of the FET and the analyte which contains the biosample. The biotin-streptavidin reaction pair is used in purification and detection of various biomolecules; the strong streptavidin-biotin bond can also be used to attach biomolecules to one another or onto a solid support. Thus this reaction pair in combination with a FET as the transducer is a powerful setup enabling the detection of a wide variety of molecules with many advantages that stem from the FET, like no labeling, no need of expensive read-out devices, the possibility to put the signal amplification and analysis on the same chip, and outdoor usage without the necessity of a lab.


  1. Bousse, L. (1982). The Chemical Sensitivity of Electrolyte/Insulator/Silicon Structures. PhD thesis.
  2. Bousse, L., Mostarshed, S., Van Der Shoot, B., De Rooij, N. F., Gimmel, P., and Gopel, W. (1991). Zeta potential measurements of Ta2O5 and SiO2 thin films. Journal of Colloid and Interface Science, 147(1):22- 32.
  3. Cui, Y., Wei, Q., Park, H., and Lieber, C. M. (2001). Nanowire nanosensors for highly sensitive and selective detection of biological and chemical species. Science, 293(5533):1289-1292.
  4. Deen, M. J. (2007). Highly sensitive, low-cost integrated biosensors. In SBCCI 2007: 20th Symposium on Integrated Circuits and System Design, page 1.
  5. Deen, M. J., Shinwari, M. W., Ranuárez, J. C., and Landheer, D. (2006). Noise considerations in fieldeffect biosensors. Journal of Applied Physics, 100(7):074703-1 -074703-8.
  6. Fritz, J., Cooper, E. B., Gaudet, S., Soger, P. K., and Manalis, S. R. (2002). Electronic detection of DNA by its intrinsic molecular charge. In PNAS, volume 99, pages 1412-1416.
  7. Gao, Z., Agarwal, A., Trigg, A., Singh, N., Fang, C., Tung, C., Fan, Y., Buddharaju, K., and Kong, J. (2007). Silicon nanowire arrays for label-free detection of DNA. Analytical Chemistry, 79(9):3291-3297.
  8. Girard, A., Bendria, F., Sagazan, O. D., Harnois, M., Bihan, F. L., Salaün, A., Mohammed-Brahim, T., Brissot, P., and Loréal, O. (2006). Transferrin electronic detector for iron disease diagnostics. IEEE Sensors, pages 474-477.
  9. Gupta, S., Elias, M., Wen, X., Shapiro, J., and Brillson, L. (2008). Detection of clinical relevant levels of protein analyte under physiologic buffer using planar field effect transistors. Biosensors and Bioelectronics, 24:505-511.
  10. Hahm, J. and Lieber, C. M. (2004). Direct ultrasensitive electrical detection of DNA and DNA sequence variations using nanowire nanosensors. Nano Letters, 4(1):51-54.
  11. Heitzinger, C., Kennell, R., Klimeck, G., Mauser, N., McLennan, M., and Ringhofer, C. (2008a). Modeling and simulation of field-effect biosensors (BioFETs) and their deployment on the nanoHUB. J. Phys.: Conf. Ser., 107:012004/1-12.
  12. Heitzinger, C. and Klimeck, G. (2007). Computational aspects of the three-dimensional feature-scale simulation of silicon-nanowire field-effect sensors for DNA detection. Journal of Computational Electronics, 6:387-390.
  13. Heitzinger, C., Mauser, N., and Ringhofer, C. (2008b). Multi-scale modeling of planar and nanowire fieldeffect biosensors. submitted.
  14. Im, H., Huang, X. ., Gu, B., and Choi, Y. . (2007). A dielectric-modulated field-effect transistor for biosensing. Nature Nanotechnology, 2(7):430-434.
  15. Kim, D., Park, J., Shin, J., Kim, P., Lim, G., and Shoji, S. (2006). An extended gate FET-based biosensor integrated with a Si microfluidic channel for detection of protein complexes. Sensors and Actuators, B: Chemical, 117:488-494.
  16. Kusnezow, W., Jacob, A., Walijew, A., Diehl, F., and Hoheisel,J.D. (2003). Antibody microarrays: An evaluation of production parameters. Proteomics, 3(3):254- 264.
  17. Landheer, D., Aers, G., McKinnon, W., Deen, M., and Ranuárez, J. (2005). Model for the field effect from layers of biological macromolecules on the gates of meta-oxide-semiconductor transistors. journal of applied physics, 98(4):044701-1 -044701-15.
  18. Oh, S. W., Moon, J. D., Lim, H. J., Park, S. Y., Kim, T., Park, J., Han, M. H., Snyder, M., and Choi, E. Y. (2005). Calixarene derivative as a tool for highly sensitive detection and oriented immobilization of proteins in a microarray format through noncovalent molecular interaction. FASEB Journal, 19(10):1335- 1337.
  19. Park, K., Lee, S., Sohn, Y., and S.Y, C. (2008). BioFET sensor for detection of albumin in urine. Electronic Letters, 44(3).
  20. Peluso, P., Wilson, D. S., Do, D., Tran, H., Venkatasubbaiah, M., Quincy, D., Heidecker, B., Poindexter, K., Tolani, N., Phelan, M., Witte, K., Jung, L. S., Wagner, P., and Nock, S. (2003). Optimizing antibody immobilization strategies for the construction of protein microarrays. Analytical Biochemistry, 312(2):113-124.
  21. Pirrung, M. C. (2002). How to make a DNA chip. Angew. Chem. Int. Ed., 41:1276-1289.
  22. Poghossian, A., Cherstvy, A., Ingebrandt, S., Offenhäusser, A., and Schöning, M. J. (2005). Possibilities and limitations of label-free detection of DNA hybridization with field-effect-based devices. Sensors and Actuators, B: Chemical, 111-112(SUPPL.):470-480.
  23. Ringhofer, C. and Heitzinger, C. (2008). Multi-scale modeling and simulation of field-effect biosensors. ECS Transactions, 14(1):11-19.
  24. Selberherr, S. (1984). Analysis and Simulation of Semiconductor Devices, volume Springer of ISBN: 3-211- 81800-6.
  25. Shinwari, M. W., Deen, M. J., and Landheer, D. (2006). Study of the elecrolyte-insulator-semiconductor fieldeffect transistor (EISFET) with applications in biosensor design. Microelectronics Reliability.
  26. Stern, E., Klemic, J., Routenberg, D., Wyrembak, P., Turner-Evans, D., Hamilton, A., LaVan, D., Fahmy, T., and Reed, M. (2007). Lable-free immunodetection with CMOS-compatible semiconducting nanowires. Nature Letters, 445(1):519-522.
  27. Tang, T.-W. and Ieong, M.-K. (1995). Discretization of flux densities in device simulations using optimum artificial diffusivity. IEEE Transactions on ComputerAided Design of Integrated Circuits and Systems, 14(11):1309-1315.
  28. Turkova, J. (1999). Oriented immobilization of biologically active proteins as a tool for revealing protein interactions and function. Journal of Chromatography B: Biomedical Sciences and Applications, 722(1-2):11- 31.
  29. Wacker, R., Schroder, H., and Niemeyer, C. M. (2004). Performance of antibody microarrays fabricated by either DNA-directed immobilization, direct spotting, or streptavidin-biotin attachment: A comparative study. Analytical Biochemistry, 330(2):281-287.
  30. Windbacher, T., Sverdlov, V., Selberherr, S., Heitzinger, C., Mauser, N., and Ringhofer, C. (2008). Simulation of field-effect biosensors (BioFETs). In Proc. Simulation of Semiconductor Processes and Devices (SISPAD 2008), pages P18/1-4, Hakone, Japan.
  31. Zheng, G., Patolsky, F., Cui, Y., Wang, W. U., and Lieber, C. M. (2005). Multiplexed electrical detection of cancer markers with nanowire sensor arrays. Nature Biotechnology, 23(10):1294-1301.

Paper Citation

in Harvard Style

Windbacher T., Sverdlov V., Selberherr S., Heitzinger C., Mauser N. and Ringhofer C. (2009). STUDY OF THE PROPERTIES OF BIOTIN-STREPTAVIDIN SENSITIVE BIOFETS . In Proceedings of the International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2009) ISBN 978-989-8111- 64-7, pages 24-30. DOI: 10.5220/0001430700240030

in Bibtex Style

author={Thomas Windbacher and Viktor Sverdlov and Siegfried Selberherr and Clemens Heitzinger and Norbert Mauser and Christian Ringhofer},
booktitle={Proceedings of the International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2009)},
isbn={978-989-8111- 64-7},

in EndNote Style

JO - Proceedings of the International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2009)
SN - 978-989-8111- 64-7
AU - Windbacher T.
AU - Sverdlov V.
AU - Selberherr S.
AU - Heitzinger C.
AU - Mauser N.
AU - Ringhofer C.
PY - 2009
SP - 24
EP - 30
DO - 10.5220/0001430700240030