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Authors: Claire Lefort ; David Moreau ; Philippe Lévêque and Rodney O'Connor

Affiliation: XLIM Institute, CNRS and Limoges University UMR 7252, France

Keyword(s): Optical Temperature Measurement, Infrared Laser Light Excitation, Evolution of Temperature, Fluorescence of Rhodamine B.

Related Ontology Subjects/Areas/Topics: Biomedical Optics ; Optical Instrumentation ; Optics ; Photodetectors, Sensors and Imaging ; Photonics ; Photonics, Optics and Laser Technology ; Spectroscopy, Imaging and Metrology

Abstract: The use of infrared laser light (IRLL) for biomedical applications has gained momentum the potential applications in humans. The use of IRLL presents some limitations due to the dangerousness of these radiations when exceeding some safety thresholds depending on the target tissue. This position paper describes step by step a well-known technical method usually applied for microfluidics but here applied for the first time to measure the temperature and the heat evolution in a cell culture environment under IRLL excitation at 800 nm. The measurement of temperature is based on the property of Rhodamine B (RhB), a fluorescent dye whose fluorescence intensity decreases linearly with temperature increases, illustrated by preliminary microscopic measurements of temperature in cell culture dishes containing RhB solution under the IRLL excitation from 0 mW to 300 mW.

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Paper citation in several formats:
Lefort, C.; Moreau, D.; Lévêque, P. and O'Connor, R. (2015). Optical Measurement of Temperature in Tissue Culture Surfaces under Infrared Laser Light Excitation at 800nm using a Fluorescent Dye. In Proceedings of the 3rd International Conference on Photonics, Optics and Laser Technology - Volume 1: PHOTOPTICS; ISBN 978-989-758-092-5; ISSN 2184-4364, SciTePress, pages 47-52. DOI: 10.5220/0005256300470052

@conference{photoptics15,
author={Claire Lefort. and David Moreau. and Philippe Lévêque. and Rodney O'Connor.},
title={Optical Measurement of Temperature in Tissue Culture Surfaces under Infrared Laser Light Excitation at 800nm using a Fluorescent Dye},
booktitle={Proceedings of the 3rd International Conference on Photonics, Optics and Laser Technology - Volume 1: PHOTOPTICS},
year={2015},
pages={47-52},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0005256300470052},
isbn={978-989-758-092-5},
issn={2184-4364},
}

TY - CONF

JO - Proceedings of the 3rd International Conference on Photonics, Optics and Laser Technology - Volume 1: PHOTOPTICS
TI - Optical Measurement of Temperature in Tissue Culture Surfaces under Infrared Laser Light Excitation at 800nm using a Fluorescent Dye
SN - 978-989-758-092-5
IS - 2184-4364
AU - Lefort, C.
AU - Moreau, D.
AU - Lévêque, P.
AU - O'Connor, R.
PY - 2015
SP - 47
EP - 52
DO - 10.5220/0005256300470052
PB - SciTePress

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