loading
Papers Papers/2022 Papers Papers/2022

Research.Publish.Connect.

Paper

Paper Unlock

Authors: Min Li ; Renrong Liu ; Lixin Zhu and Yu Jin

Affiliation: School of Life Science, Jiangxi Science & Technology Normal University, Jiangxi, Nanchang, 330013 and China, China

Keyword(s): Aflatoxin M1, chemiluminescence enzyme immunoassay, high performance liquid chromatography

Abstract: A rapid and sensitive chemiluminescence enzyme immunoassay method (CLEIA) was established to detect Aflatoxin M1 (AFM1) in milk, which was Verified by high performance liquid chromatography (HPLC). It only takes 30 minutes. Optimized conditions included antibody dilution ratio and enzyme conjugate, ionic strength, pH value and organic solvent. Results: The 50% inhibitory concentration(IC50) and the detection limit of the CLEIA were 0.08ng/mL and 0.024ng/mL, the recovery ranged from 86.94% to 114.49% in dairy products. The correlation was 99.7% between this method and HPLC .

CC BY-NC-ND 4.0

Sign In Guest: Register as new SciTePress user now for free.

Sign In SciTePress user: please login.

PDF ImageMy Papers

You are not signed in, therefore limits apply to your IP address 3.239.119.159

In the current month:
Recent papers: 100 available of 100 total
2+ years older papers: 200 available of 200 total

Paper citation in several formats:
Li, M.; Liu, R.; Zhu, L. and Jin, Y. (2019). Establishment of a Rapid and Sensitive Chemiluminescence Enzyme Immunoasay for Aflatoxin M1: Verified by HPLC. In The Second International Conference on Materials Chemistry and Environmental Protection - MEEP; ISBN 978-989-758-360-5, SciTePress, pages 51-54. DOI: 10.5220/0008185300510054

@conference{meep19,
author={Min Li. and Renrong Liu. and Lixin Zhu. and Yu Jin.},
title={Establishment of a Rapid and Sensitive Chemiluminescence Enzyme Immunoasay for Aflatoxin M1: Verified by HPLC},
booktitle={The Second International Conference on Materials Chemistry and Environmental Protection - MEEP},
year={2019},
pages={51-54},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0008185300510054},
isbn={978-989-758-360-5},
}

TY - CONF

JO - The Second International Conference on Materials Chemistry and Environmental Protection - MEEP
TI - Establishment of a Rapid and Sensitive Chemiluminescence Enzyme Immunoasay for Aflatoxin M1: Verified by HPLC
SN - 978-989-758-360-5
AU - Li, M.
AU - Liu, R.
AU - Zhu, L.
AU - Jin, Y.
PY - 2019
SP - 51
EP - 54
DO - 10.5220/0008185300510054
PB - SciTePress