loading
Documents

Research.Publish.Connect.

Paper

Paper Unlock

Authors: Nicola Moscelli 1 ; Sander van den Driesche 1 ; Michael J. Vellekoop 1 and Wojciech Witarski 2

Affiliations: 1 Vienna University of Technology, Austria ; 2 Slovak Academy of Sciences, Slovak Republic

ISBN: 978-989-8425-37-9

Keyword(s): Optical detection, CCD sensor, Real-time monitoring, Epithelial cells.

Related Ontology Subjects/Areas/Topics: Biomedical Engineering ; Biomedical Instruments and Devices ; Biomedical Sensors ; Imaging and Visualization Devices

Abstract: In this contribution we present a compact imaging system to monitor the proliferation and the motility of cells in real-time. Our monitoring system is compatible with standard multi-well plates and operates in CO2, temperature and humidity controlled cell-culture incubators. Adherent grown epithelial cells in a multi-well plate well, positioned on top of a custom made holder, have been monitored in real-time with a fixed CCD image sensor. As light source an LED is placed above the plate holder. A field of view of 3.3 × 2.5 mm2 was achieved by using a 4.6 × 4.0 mm2 image sensor and mini lens system. The image sensor has a resolution of 640 × 480 pixels. Consequently, the obtained sensing resolution of the imaging system is about 5 μm. The cell monitoring system has first been validated by visualizing micro-beads of known dimensions. Then, our system has been successfully tested, tracking the migration paths and proliferation of respectively adherent grown MDCK (Madin-Darby canine kidne y) and A549 (human lung carcinoma) epithelial cells. (More)

PDF ImageFull Text

Download
Sign In Guest: Register as new SciTePress user now for free.

Sign In SciTePress user: please login.

PDF ImageMy Papers

You are not signed in, therefore limits apply to your IP address 3.208.22.127

In the current month:
Recent papers: 100 available of 100 total
2+ years older papers: 200 available of 200 total

Paper citation in several formats:
Moscelli, N.; van den Driesche, S.; Vellekoop, M. and Witarski, W. (2011). A REAL-TIME CELL PROLIFERATION AND MOTILITY MONITORING SYSTEM.In Proceedings of the International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2011) ISBN 978-989-8425-37-9, pages 230-233. DOI: 10.5220/0003167002300233

@conference{biodevices11,
author={Nicola Moscelli. and Sander van den Driesche. and Michael J. Vellekoop. and Wojciech Witarski.},
title={A REAL-TIME CELL PROLIFERATION AND MOTILITY MONITORING SYSTEM},
booktitle={Proceedings of the International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2011)},
year={2011},
pages={230-233},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0003167002300233},
isbn={978-989-8425-37-9},
}

TY - CONF

JO - Proceedings of the International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2011)
TI - A REAL-TIME CELL PROLIFERATION AND MOTILITY MONITORING SYSTEM
SN - 978-989-8425-37-9
AU - Moscelli, N.
AU - van den Driesche, S.
AU - Vellekoop, M.
AU - Witarski, W.
PY - 2011
SP - 230
EP - 233
DO - 10.5220/0003167002300233

Login or register to post comments.

Comments on this Paper: Be the first to review this paper.