Authors:
Massimo Natale
1
;
Alfonso Caiazzo
2
;
Enrico M. Bucci
3
and
Elisa Ficarra
4
Affiliations:
1
Politecnico di Torino and BioDigitalValley srl, Italy
;
2
WIAS Berlin, Germany
;
3
BioDigitalValley srl and Istituto di Biostrutture e Bioimmagini, Italy
;
4
Politecnico di Torino, Italy
Keyword(s):
Image analysis, Bi-dimensional electrophoresis, Proteomics, Software tools.
Related
Ontology
Subjects/Areas/Topics:
Algorithms and Software Tools
;
Bioinformatics
;
Biomedical Engineering
;
Image Analysis
Abstract:
Analysis of 2D-GE images is a hot topic in bioinformatics research, since currently available commercial and academic software has proven to be not really effective and not completely automatic, often requiring manual revision of spots detection and refinement of computer generated matches. In this work, we present an effective technique for the detection and the reconstruction of over-saturated protein spots.
Firstly, it reveals overexposed areas where spots may be truncated, and plateau regions caused by smeared and overlapped spots. As next, the correct distribution of pixel values in the overexposed areas and plateau regions is recovered by a two-dimensional fitting based on a generalized Gaussian distribution approximating the spots volume. Pixel correction according to the generalized Gaussian curve in saturated and smeared spots allows more accurate quantifications, providing more reliable image analysis results.
As validation, we process highly exposed 2D-GE image, containi
ng saturate spots, with respect to the corre-sponding non-saturated image, confirming that the method can effectively fix the saturated spots and enable correct spots quantification.
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