Authors:
Bohuslav Rezek
1
;
Lenka Michalíková
2
;
Egor Ukraintsev
2
;
Alexander Kromka
2
and
Marie Kalbacova
3
Affiliations:
1
Institute of Physics ASCR, v.v.i., Czech Republic
;
2
Academy of Sciences of the Czech Republic, Czech Republic
;
3
1st Faculty of Medicine, Charles University, Czech Republic
Keyword(s):
Cell adhesion, Proteins, Diamond, Biotechnology, Biosensors, Osteoblasts, Atomic force microscopy.
Related
Ontology
Subjects/Areas/Topics:
Biomaterials
;
Biomedical Engineering
;
Biomedical Instruments and Devices
;
Biomedical Sensors
;
Nanotechnologies
Abstract:
Unique combination of chemical and biocompatible properties with semiconducting properties makes diamond an attractive material for merging solid state and biological systems. Microscopic chemical patterning of diamond films by hydrogen and oxygen surface atoms is used for self-assembly of human osteoblastic cells into micro-arrays. The cell adhesion and assembly on the diamond is further controlled and optimized by cell and protein (fetal bovine serum - FBS) concentration. The cells are characterized by fluorescence microscopy of actin fibers and nuclei. The protein adsorption is studied by atomic force microscopy (AFM). The cells are arranged into arrays on O-terminated patterns. The best cell selectivity is achieved for the lowest cell concentrations of 2500 cells/cm2. Higher cell concentrations enable to colonize unfavorable H-terminated regions due to mutual cell communication. Based on AFM, the proteins are present on both H- and O-terminated surfaces, however, pronounced diffe
rences in the thickness, surface roughness, morphology, and phase images indicate different conformation of the proteins and hence the cell selectivity. There is no cell selectivity when no protein is supplemented in the medium. These results may be applicable in tissue engineering, implants, bio-electronics, and biotechnology in general.
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