loading
Papers

Research.Publish.Connect.

Paper

Paper Unlock

Authors: Bohuslav Rezek 1 ; Lenka Michalíková 2 ; Egor Ukraintsev 2 ; Alexander Kromka 2 and Marie Kalbacova 3

Affiliations: 1 Institute of Physics ASCR, v.v.i., Czech Republic ; 2 Academy of Sciences of the Czech Republic, Czech Republic ; 3 1st Faculty of Medicine, Charles University, Czech Republic

ISBN: 978-989-8111- 64-7

Keyword(s): Cell adhesion, Proteins, Diamond, Biotechnology, Biosensors, Osteoblasts, Atomic force microscopy.

Related Ontology Subjects/Areas/Topics: Biomaterials ; Biomedical Engineering ; Biomedical Instruments and Devices ; Biomedical Sensors ; Nanotechnologies

Abstract: Unique combination of chemical and biocompatible properties with semiconducting properties makes diamond an attractive material for merging solid state and biological systems. Microscopic chemical patterning of diamond films by hydrogen and oxygen surface atoms is used for self-assembly of human osteoblastic cells into micro-arrays. The cell adhesion and assembly on the diamond is further controlled and optimized by cell and protein (fetal bovine serum - FBS) concentration. The cells are characterized by fluorescence microscopy of actin fibers and nuclei. The protein adsorption is studied by atomic force microscopy (AFM). The cells are arranged into arrays on O-terminated patterns. The best cell selectivity is achieved for the lowest cell concentrations of 2500 cells/cm2. Higher cell concentrations enable to colonize unfavorable H-terminated regions due to mutual cell communication. Based on AFM, the proteins are present on both H- and O-terminated surfaces, however, pronounced differ ences in the thickness, surface roughness, morphology, and phase images indicate different conformation of the proteins and hence the cell selectivity. There is no cell selectivity when no protein is supplemented in the medium. These results may be applicable in tissue engineering, implants, bio-electronics, and biotechnology in general. (More)

PDF ImageFull Text

Download
CC BY-NC-ND 4.0

Sign In Guest: Register as new SciTePress user now for free.

Sign In SciTePress user: please login.

PDF ImageMy Papers

You are not signed in, therefore limits apply to your IP address 34.237.51.159

In the current month:
Recent papers: 100 available of 100 total
2+ years older papers: 200 available of 200 total

Paper citation in several formats:
Rezek B.; Michalíková L.; Ukraintsev E.; Kromka A.; Kalbacova M. and (2009). SELECTIVE OSTEOBLASTIC CELL MICRO-ARRAYS ON DIAMOND FILMS.In Proceedings of the International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2009) ISBN 978-989-8111- 64-7, pages 347-354. DOI: 10.5220/0001434203470354

@conference{biodevices09,
author={Bohuslav Rezek and Lenka Michalíková and Egor Ukraintsev and Alexander Kromka and Marie Kalbacova},
title={SELECTIVE OSTEOBLASTIC CELL MICRO-ARRAYS ON DIAMOND FILMS},
booktitle={Proceedings of the International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2009)},
year={2009},
pages={347-354},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0001434203470354},
isbn={978-989-8111- 64-7},
}

TY - CONF

JO - Proceedings of the International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2009)
TI - SELECTIVE OSTEOBLASTIC CELL MICRO-ARRAYS ON DIAMOND FILMS
SN - 978-989-8111- 64-7
AU - Rezek, B.
AU - Michalíková, L.
AU - Ukraintsev, E.
AU - Kromka, A.
AU - Kalbacova, M.
PY - 2009
SP - 347
EP - 354
DO - 10.5220/0001434203470354

Login or register to post comments.

Comments on this Paper: Be the first to review this paper.