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Authors: Wahyu Hidayati ; Ade Nur Padillah ;  Maharadingga ; Ni Putu Ermi Hikmawanti ; Rini Prastiwi ; Ani Pratiwi ; Lady Farahmayuni ; Rezza Syahputra and Muhammad Fahrul

Affiliation: Universitas Muhammadiyah , Indonesia

Keyword(s): Eugenia polyantha, Endophytic fungi, Antidiabetic, Alpha-amylase, Inhibition, Diabetes

Abstract: Indonesian people use bay leaves as spices in local culinary and as traditional medicine, particularly to treat diabetes. The problems with the mass production of antidiabetic drugs from bay leaves can be solved by utilizing endophytic fungi as an alternative source for antidiabetic compounds. This study aimed to isolate endophytic fungi from bay leaves and identify their antidiabetic activity through the in vitro inhibition of alpha-amylase. The leaves were processed on potato dextrose agar media, and five isolates were grown in an agar medium. The fermentation used a potato dextrose yeast medium that was left for five days on an orbital shaker at room temperature. The crude was extracted using ethyl acetate solvent. In the in vitro alpha-amylase inhibition test, the antidiabetic assay used the ethyl acetate extract of the endophytic fungi. The inhibition percentage was calculated from the absorbance value read by a microplate reader. All isolates inhibited alphaamylase activity, bu t only three of them had high inhibition percentages (14.385%, 12.849%, and 39.246%). As a conclusion, the endophytic fungi isolated from bay leaves are potential as an alternative source for the production of secondary metabolites to cure diabetes. (More)

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Paper citation in several formats:
Hidayati, W.; Padillah, A.; Maharadingga.; Hikmawanti, N.; Prastiwi, R.; Pratiwi, A.; Farahmayuni, L.; Syahputra, R. and Fahrul, M. (2019). The Alpha-Amylase Inhibition Potential of Endophytic Fungi from Indonesian Bay Leaves (Eugenia polyantha WIGHT.). In Proceedings of the 1st Muhammadiyah International Conference on Health and Pharmaceutical Development - MICH-PhD; ISBN 978-989-758-349-0, SciTePress, pages 107-111. DOI: 10.5220/0008240201070111

@conference{mich-phd19,
author={Wahyu Hidayati. and Ade Nur Padillah. and Maharadingga. and Ni Putu Ermi Hikmawanti. and Rini Prastiwi. and Ani Pratiwi. and Lady Farahmayuni. and Rezza Syahputra. and Muhammad Fahrul.},
title={The Alpha-Amylase Inhibition Potential of Endophytic Fungi from Indonesian Bay Leaves (Eugenia polyantha WIGHT.)},
booktitle={Proceedings of the 1st Muhammadiyah International Conference on Health and Pharmaceutical Development - MICH-PhD},
year={2019},
pages={107-111},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0008240201070111},
isbn={978-989-758-349-0},
}

TY - CONF

JO - Proceedings of the 1st Muhammadiyah International Conference on Health and Pharmaceutical Development - MICH-PhD
TI - The Alpha-Amylase Inhibition Potential of Endophytic Fungi from Indonesian Bay Leaves (Eugenia polyantha WIGHT.)
SN - 978-989-758-349-0
AU - Hidayati, W.
AU - Padillah, A.
AU - Maharadingga.
AU - Hikmawanti, N.
AU - Prastiwi, R.
AU - Pratiwi, A.
AU - Farahmayuni, L.
AU - Syahputra, R.
AU - Fahrul, M.
PY - 2019
SP - 107
EP - 111
DO - 10.5220/0008240201070111
PB - SciTePress

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