Authors:
Ning Wei
;
Ran Xu
and
Rongzhi Tang
Affiliation:
State Key Laboratory of Pollution Control and Resources Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, PR China, Shanghai Institute of Pollution Control and Ecological Security, Shanghai200092 and P.R. China, China
Keyword(s):
2,4-Dichlorophenol, Horseradish Peroxidase Immobilization, Electrospinning, Nanofibrous Membrane, Water Treatment
Abstract:
In this study, a facile and simple method was used to modify the hydrophobic PAN/PVdF membranes into hydrophilic ones. Results showed that the PAN/PVdF membranes have been modified successfully and the water contact angle changed from 87.135° to 0°. Horseradish peroxidase (HRP) was immobilized onto the modified PAN/PVdF membranes through covalent binding and the maximum enzyme loading was approximately 440 mg/g under optimal conditions (after 8 h at pH 8.0 and 25 oC). The effects of pH and temperature on the relative activity of free and immobilized HRP were studied. Under the optimum conditions of pH and temperature, immobilized HRP was greatly improved in operational and storage stability. 2,4-DCP removal experiments showed that the immobilized HRP and free HRP had a similar removal efficiency (87% and 93%, respectively). However, the immobilized HRP had an excellent reusability. HRP-PAN/PVdF could still remove 47.6% of 2,4-DCP after 7 repeated runs, which could overcome the inhere
nt drawbacks of free enzymes-hard separation and non-reusability.
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