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Authors: M. Zanello 1 ; A. Ibrahim 2 ; F. Poulon 2 ; P. Varlet 3 ; B. Devaux 4 and D. Abi Haidar 5

Affiliations: 1 IMNC Laboratory, UMR 8165-CNRS, Department of Neurosurgery and Sainte Anne Hospital, France ; 2 IMNC Laboratory and UMR 8165-CNRS, France ; 3 Department of Neuropathology and Sainte Anne Hospital, France ; 4 Department of Neurosurgery and Sainte Anne Hospital, France ; 5 IMNC Laboratory, UMR 8165-CNRS and University of Paris Diderot - Paris 7, France

Keyword(s): Lifetime Measurement, Spectroscopy, Autofluorescence, Metastasis and Cortical Human Samples.

Related Ontology Subjects/Areas/Topics: Biomedical Optics ; Optics ; Photonics, Optics and Laser Technology ; Spectroscopy, Imaging and Metrology

Abstract: Analysis of human tissue endogenous fluorescence is becoming a new modality of medical imaging. Its capacities represent the missing link between macroscopic radiological tools such as MRI and CT-scan and the surgeon view during surgical procedures. However, numerous aspects of this signal are not well known. Time dependence is one of these aspects. The aim of this work is to investigate the autofluorescence changes with time. Five ex vivo human samples were studied. Spectral and lifetime measurements were acquired each hour. Fluorescence intensity decreased slightly with time. This decrease existed for healthy and tumoral samples and did not affect the differences between them: higher fluorescence intensity for control samples compared to tumor samples. Lifetime values showed a slight decrease too for both type of tissue. This work is the first report of fresh human brain samples multimodal autofluorescence analysis with time.

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Paper citation in several formats:
Zanello, M.; Ibrahim, A.; Poulon, F.; Varlet, P.; Devaux, B. and Abi Haidar, D. (2016). Spectral and Lifetime Measurements of the Endogenous Fluorescence Variation of Freshly Resected Human Samples over Time - Measuring Endogenous Fluorescence Changes at Different Moment after Tumor or Epileptic Cortex Excision. In Proceedings of the 4th International Conference on Photonics, Optics and Laser Technology - PHOTOPTICS; ISBN 978-989-758-174-8; ISSN 2184-4364, SciTePress, pages 13-17. DOI: 10.5220/0005654900130017

@conference{photoptics16,
author={M. Zanello. and A. Ibrahim. and F. Poulon. and P. Varlet. and B. Devaux. and D. {Abi Haidar}.},
title={Spectral and Lifetime Measurements of the Endogenous Fluorescence Variation of Freshly Resected Human Samples over Time - Measuring Endogenous Fluorescence Changes at Different Moment after Tumor or Epileptic Cortex Excision},
booktitle={Proceedings of the 4th International Conference on Photonics, Optics and Laser Technology - PHOTOPTICS},
year={2016},
pages={13-17},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0005654900130017},
isbn={978-989-758-174-8},
issn={2184-4364},
}

TY - CONF

JO - Proceedings of the 4th International Conference on Photonics, Optics and Laser Technology - PHOTOPTICS
TI - Spectral and Lifetime Measurements of the Endogenous Fluorescence Variation of Freshly Resected Human Samples over Time - Measuring Endogenous Fluorescence Changes at Different Moment after Tumor or Epileptic Cortex Excision
SN - 978-989-758-174-8
IS - 2184-4364
AU - Zanello, M.
AU - Ibrahim, A.
AU - Poulon, F.
AU - Varlet, P.
AU - Devaux, B.
AU - Abi Haidar, D.
PY - 2016
SP - 13
EP - 17
DO - 10.5220/0005654900130017
PB - SciTePress