Authors:
Min Cheol Park
1
;
Moon Kyu Kwak
1
;
Hye Sung Cho
1
;
Kahp Y. Suh
1
;
Jae Young Hur
2
and
Sang-Hyun Park
2
Affiliations:
1
School of Mechanical and Aerospace Engineering, Seoul National University, Korea, Republic of
;
2
Seoul National University, Korea, Republic of
Keyword(s):
Cell chip, single-cell, MAPK signaling pathway, stochastic kinetics, receding meniscus, florescent protein.
Related
Ontology
Subjects/Areas/Topics:
Biocomputing and Biochips
;
Biomechanical Devices
;
Biomedical Engineering
;
Biomedical Instruments and Devices
;
Biomedical Sensors
;
Hardware
;
MEMS
;
Nanotechnologies
;
Sensor Networks
Abstract:
The mitogen-activated protein kinase (MAPK) signaling pathways are essential for cell growth, cell differentiation and survival in eukaryotes. The MAPK signaling pathways transmit signals from the cell surface to nucleus. The mating and high osmolarity responses in the budding yeast, Saccharomyces cerevisiae, depend on the MAPK signaling pathways. Here we analyzed the mating and high osmolarity responses in the budding yeast, S. cerevisiae at single-cell level using cell chips. The cell chip analyses of the mating and high osmolarity responses were performed using fluorescent proteins fused to genes whose transcription is specifically upregulated by each signaling. Using the technique, we have determined the real-time gene expression patterns of the mating and high osmolarity responses at single-cell level. In this study, we observed that the mating and high osmolarity MAPK signaling showed a non-uniform, fluctuating flux in the population of yeast cells analyzed.