Authors:
Marine Astruc
1
;
Patrik Malm
2
;
Rajesh Kumar
3
and
Ewert Bengtsson
2
Affiliations:
1
Ecole Centrale Nantes, France
;
2
Uppsala University, Sweden
;
3
Centre for Development of Advanced Computing, India
Keyword(s):
Pap-smear, Automated Screening, Cluster Detection, Field-of-View Analysis.
Related
Ontology
Subjects/Areas/Topics:
Applications
;
Clustering
;
Computer Vision, Visualization and Computer Graphics
;
Graphical and Graph-Based Models
;
Image Understanding
;
Medical Imaging
;
Pattern Recognition
;
Software Engineering
;
Theory and Methods
Abstract:
Automated cervical cancer screening systems require high resolution analysis of a large number of epithelial cells, involving complex algorithms, mainly analysing the shape and texture of cell nuclei. This can be a very time consuming process. An initial selection of relevant fields-of-view in low resolution images could limit the number of fields to be further analysed at a high resolution. In particular, the detection of cell clusters is of interest for nuclei segmentation improvement, and for diagnostic purpose, malignant and endometrial cells being more prone to stick together in clusters than other cells. In this paper, we propose methods aiming at evaluating the quality of fields-of-view in bright-field microscope images of cervical cells. The approach consists of the construction of neighbourhood graphs using the nuclei as the set of vertices. Transformations are then applied to such graphs in order to highlight the main structures in the image. The methods result in the delin
eation of regions with varying cell density and the identification of cell clusters. Clustering methods are evaluated using a dataset of manually delineated clusters and compared to a related work.
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