Authors:
Daniela Woide
1
;
Veronika Schlentner
1
;
Teresa Neumaier
1
;
Thorsten Wachtmeister
1
;
Herwig G. Paretzke
1
;
Zeno von Guttenberg
2
;
Achim Wixforth
3
and
Stefan Thalhammer
1
Affiliations:
1
GSF, National Research Center for Environment and Health, Institute of Radiation Protection, Germany
;
2
Advalytix AG, Germany
;
3
University Augsburg, Chair for Experimental Physics I, Germany
Keyword(s):
Nanobiotechnology, lab-on-a-chip, cytogenetics, microfluidic PCR, surface acoustic waves, laser-based microdissection.
Related
Ontology
Subjects/Areas/Topics:
Biocomputing and Biochips
;
Biomedical Engineering
;
Biomedical Instruments and Devices
;
Nanotechnologies
Abstract:
This project focuses on the development of an acoustic driven, freely programmable multifunctional biochemical
lab-on-a-chip. By combining different platform elements, like microdissection-, nanofluidic- and detection-modules, the lab-on-a-chip can be adapted to question- and patient-specific cytogenetic and forensic applications. In contrast to many common lab-on-a-chip approaches presently available, the fluidic handling is done on a planar surface of the lab-on-a-chip. Minute amounts of biochemical fluids are confined in ‘virtual’ reaction chambers and ‘virtual’ test tubes in the form of free droplets. The droplets, fluidic tracks and reaction sites are defined at the chip surface by a monolayer chemical modification of the chip surface. Surface acoustic waves are employed to agitate and actuate these little ‘virtual’ test tubes along predetermined trajectories. Well-defined investigations, controlled in the submicrolitre regime, can be conducted quickly and gently on the lab-on
-a-chip.
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