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Authors: Peter Peer 1 and Luis Galo Corzo 2

Affiliations: 1 CEIT and Tecnun (University of Navarra); Faculty of Computer and Information Science, University of Ljubljana, Slovenia ; 2 CEIT and Tecnun (University of Navarra); Asiris Vision Technologies, Spain

ISBN: 978-972-8865-73-3

Keyword(s): Image analysis, two-dimensional gel electrophoresis, segmentation.

Related Ontology Subjects/Areas/Topics: Computer Vision, Visualization and Computer Graphics ; Feature Extraction ; Features Extraction ; Image and Video Analysis ; Informatics in Control, Automation and Robotics ; Segmentation and Grouping ; Signal Processing, Sensors, Systems Modeling and Control

Abstract: Two-dimensional gel electrophoresis (2-DE) images show the expression levels of several hundred of proteins where each protein is represented as a blob shaped spot of grey level values. The spot detection, i.e. segmentation process has to be efficient as it is the first step in the gel processing. Such extraction of information is a very complex task. In this paper we propose a real time spot detector that is basically a morphology based method with use of seeded region growing as a central paradigm and which relies on the spot correlation information. The method is tested on gels with human samples in SWISS-2DPAGE (two-dimensional polyacrylamide gel electrophoresis) database. The average time to process the image is less than a second, while the results are very intuitive for human perception and as such they help the user to focus on important parts of the gel in the subsequent processing. In gels with less than 50 identified spots as proteins (proteins that compose a proteome) in t he mentioned database, the algorithm detects all obvious spots. (More)

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Paper citation in several formats:
Peer P.; Galo Corzo L. and (2007). FAST SPOT HYPOTHESIZER FOR 2-DE RESEARCH.In Proceedings of the Second International Conference on Computer Vision Theory and Applications - Volume 1: VISAPP, ISBN 978-972-8865-73-3, pages 284-289. DOI: 10.5220/0002050002840289

@conference{visapp07,
author={Peter Peer and Luis {Galo Corzo}},
title={FAST SPOT HYPOTHESIZER FOR 2-DE RESEARCH},
booktitle={Proceedings of the Second International Conference on Computer Vision Theory and Applications - Volume 1: VISAPP,},
year={2007},
pages={284-289},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0002050002840289},
isbn={978-972-8865-73-3},
}

TY - CONF

JO - Proceedings of the Second International Conference on Computer Vision Theory and Applications - Volume 1: VISAPP,
TI - FAST SPOT HYPOTHESIZER FOR 2-DE RESEARCH
SN - 978-972-8865-73-3
AU - Peer, P.
AU - Galo Corzo, L.
PY - 2007
SP - 284
EP - 289
DO - 10.5220/0002050002840289

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