Authors: Adlina Savira 1 ; Achmad Syahrani 1 and Marcellino Rudyanto 2

Affiliations: 1 Universitas Airlangga, Indonesia ; 2 Universitas Airlangga, Institute of Tropical Disease and Universitas Airlangga, Indonesia

ISBN: 978-989-758-347-6

Keyword(s): citral, Cymbopogon citratus, chromatograhy, separation

Abstract: Background. Citral is the main component of lemongrass (Cymbopogon citratus) oil. This compound has biological activities such as antibacterial, antifungi, analgesic, and antiinflammation. Citral also has importance for its use as starting material for the synthesis of Vitamin A. Due to the broad utilisation of citral, it is important to develop method of isolation which is relatively simple, low cost, but able to give pure citral in high yield. Materials and method. Citral was isolated from commercially available lemongrass oil by simple column chromatography using silica gel as stationary phase. Elution was carried out in isoctaric mode. Mobile phase was chosen among hexane – diethyl ether, hexane – ethyl acetate, and hexane – ethanol based on separation factor and Rf value on thin layer chromatography. Optimum ratio of sample and stationary phase was also optimized based on isolation yield. Isolated citral was analyzed by gas chromatography – mass spectroscopy. Results. The best se paration factor on TLC was obtained from hexane – ethyl acetate (97:3) as eluent. The best yield (49,61 ± 2,59 %) was obtained when stationary phase was used at ratio 20:1 to sample. (More)

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Paper citation in several formats:
Savira, A.; Syahrani, A. and Rudyanto, M. (2018). A Simple Method for Isolation of Citral Using Column Chromatography.In Proceedings of BROMO Conference - Volume 1: BROMO, ISBN 978-989-758-347-6, pages 12-20. DOI: 10.5220/0008356900120020

author={Adlina Savira. and Achmad Syahrani. and Marcellino Rudyanto.},
title={A Simple Method for Isolation of Citral Using Column Chromatography},
booktitle={Proceedings of BROMO Conference - Volume 1: BROMO,},


JO - Proceedings of BROMO Conference - Volume 1: BROMO,
TI - A Simple Method for Isolation of Citral Using Column Chromatography
SN - 978-989-758-347-6
AU - Savira, A.
AU - Syahrani, A.
AU - Rudyanto, M.
PY - 2018
SP - 12
EP - 20
DO - 10.5220/0008356900120020

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