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Authors: Enrique Valera 1 ; Ángel Rodríguez 1 ; Javier Ramón-Azcón 2 ; Francisco J. Sanchez 2 and M.-Pilar Marco 2

Affiliations: 1 Universitat Politècnica de Catalunya, Spain ; 2 Applied Molecular Receptors Group (AMRg), IIQAB-CSIC CIBER of Bioengineering, Biomaterials and Nanomedicine, Spain

Keyword(s): Immunosensor; Interdigitated μ-electrodes, Atrazine; Impedance spectroscopy, Conductive measurements, Wine matrix effect, Food safety.

Related Ontology Subjects/Areas/Topics: Biomedical Engineering ; Biomedical Instruments and Devices ; Biomedical Sensors ; Electrical Bio-Impedance ; Microelectronics ; Nanotechnologies

Abstract: Two novel immunosensors, one impedimetric and other one conductimetric, for atrazine detection in red wine samples have been developed. Impedimetric immunosensor is based on an array of interdigitated μ-electrodes (IDµEs) and bioreagents specifically developed to detect this pesticide. Conductimetric immunosensor incorporates additionally gold nanoparticles. Bioreagents were covalently immobilized on the surface of the electrodes (interdigital space). In both cases the biochemical determination of atrazine is possible without any redox mediator. For the case of the impedimetric immunosensor, the detection method is based on impedimetric measurements (in a wide range of frequencies), whereas in the case of the conductimetric immunosensor the detection method is based on conductimetric measurements (DC measurements).The potential of the impedimetric immunosensor to analyze atrazine in complex sample matrices, such as red wine, have been evaluated. This immunosensor can detect atrazine with limits of detection in the order sub-ppb, far below the maximum residue level (MRL) (50 µg L−1) established by European Union (EU) for residues of this herbicide in the wine grapes. (More)

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Paper citation in several formats:
Valera, E.; Rodríguez, Á.; Ramón-Azcón, J.; J. Sanchez, F. and Marco, M. (2009). IMMUNOSENSORS FOR ATRAZINE DETECTION IN RED WINE SAMPLES . In Proceedings of the International Conference on Biomedical Electronics and Devices (BIOSTEC 2009) - BIODEVICES; ISBN 978-989-8111- 64-7; ISSN 2184-4305, SciTePress, pages 226-230. DOI: 10.5220/0001542002260230

@conference{biodevices09,
author={Enrique Valera. and Ángel Rodríguez. and Javier Ramón{-}Azcón. and Francisco {J. Sanchez}. and M.{-}Pilar Marco.},
title={IMMUNOSENSORS FOR ATRAZINE DETECTION IN RED WINE SAMPLES },
booktitle={Proceedings of the International Conference on Biomedical Electronics and Devices (BIOSTEC 2009) - BIODEVICES},
year={2009},
pages={226-230},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0001542002260230},
isbn={978-989-8111- 64-7},
issn={2184-4305},
}

TY - CONF

JO - Proceedings of the International Conference on Biomedical Electronics and Devices (BIOSTEC 2009) - BIODEVICES
TI - IMMUNOSENSORS FOR ATRAZINE DETECTION IN RED WINE SAMPLES
SN - 978-989-8111- 64-7
IS - 2184-4305
AU - Valera, E.
AU - Rodríguez, Á.
AU - Ramón-Azcón, J.
AU - J. Sanchez, F.
AU - Marco, M.
PY - 2009
SP - 226
EP - 230
DO - 10.5220/0001542002260230
PB - SciTePress