Authors:
Hayate Goto
1
;
Nofel Lagrosas
2
and
Tatsuo Shiina
1
Affiliations:
1
Chiba University, Yayoi-cho, Inage-ku, Chiba-shi, Chiba, Japan
;
2
School of Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka, Japan
Keyword(s):
OCT, Indicator Plant, Environmental Assessment, Ozone, GLCM.
Abstract:
Changes in environmental conditions can be evaluated by detecting the conditions in indicator plants. Indicator plants are sensitive to specific environmental stresses. This research focused on white clover as an indicator plant for ozone. To analyze the effects of weaker stresses, compact OCT (Optical coherence tomography) for plants was developed, which allows for non-invasive and non-contact cross-sectional imaging of white clover (Trifolium repens) leaves exposed to ozone gas. OCT image changes on each level of ozone damage were evaluated using parameters such as the OCT signal level of the leaf palisade layer, the thickness of the leaf palisade layer, and texture analysis using GLCM (Gray-Level Co-occurrence Matrix). Measurements of leaves grown in our laboratory showed increased palisade tissue signal, thicker palisade tissue, a smaller distribution of palisade layer thickness, increased OCT image contrast, and decreased OCT image homogeneity.