Authors:
Adi Yugatama
;
Sholichah Rohmani
and
Rizky Apriliani
Affiliation:
Sebelas Maret University, Indonesia
Keyword(s):
eugenol, ointment, galangal rhizome, UV-Vis spectrophotometry
Abstract:
Galangal (Alpinia galanga L.) is a plant that commonly used by people as traditional medicines. Galangal rhizome contains various compounds such as galangin, methyl cinnamate, cineole, camphor, δ-pinene, and eugenol. Eugenol has an analgesic, antioxidant, and antibacterial activity that is usually used in topical preparations. In manufactured drugs, it is necessary to examine active compound which is one of the requirements to ensure its quality. This study aims to find out eugenol levels in the ointment of ethanolic extract from galangal rhizome. The method used to extract secondary metabolite from galangal rhizome is digestion using ethanol 70%. Eugenol separation from ointment of ethanolic extract of galangal rhizome was done by liquid-liquid extraction using chloroform as a solvent. The eugenol was analyzed using a UV-Vis Spectrophotometer because eugenol has chromophore, a benzene ring, so able to absorb ultraviolet light. Parameters of validation method used in this study are li
nearity, accuracy, precision, Limit of Detection (LOD) and Limit of Quantification (LOQ). The result showed that analysis method of eugenol in the ointment of ethanolic extract of galangal rhizome has good validation, with linearity, accuracy and precision occupied the requirement, LOD level is 2.388 μg/mL and LOQ level 7.235 μg/mL. Determination of eugenol level in the ointment of ethanolic extract from galangal rhizome obtained the result of 5.187 mg/gr sample.
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