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Authors: Xiaojun Deng 1 ; Jian Tang 1 ; Guoying Zhou 2 and Jizhao Cao 1

Affiliations: 1 Guangxi Zhuang Autonomous Region Fotestry Research Institute, Guangxi Key Laboratory of Superior Timber Trees Resource Cultivation, Key Laboratory of Central South Fast-growing Timber Cultivation of Forestry Ministry of China, Nanning 530002, Guangxi and Chi, China ; 2 Central South University of Forestry &Technology, Changsha 410004, Hunan and China, China

Keyword(s): N+ implantation, Beauveria bassiana, Mutation breeding, Biston marginata.

Abstract: Low-energy ion implantation technology was used to generate mutants of the high virulence Beauveria bassiana strain BbIII and bioassays were carried out on Biston marginata. The survival rate curve of B. bassiana to doses of N+ showed a “saddle shape.” The peak value 15 × 1014 ions·cm-2 was the optimal dose for mutagenesis. The growth characteristics of B. bassiana, such as colony morphology, sporulation, and spore germination rate, were affected by N+ implantation. Three stains of B. bassiana were selected based on sporulation, chitinase, and Pr1 enzyme activity as the screening indices. The activities of chitinase and Pr1 enzyme in Bb III 22 were 0.230 and 0.137 (OD value), and these were almost twice that of the original strain. The Bb III 22 strain was the most virulent to B. marginata and the mortality was > 86.7% at a concentration of 107 spores·mL-1. The log LD50 of the mutant strain spores against B. marginata was 5.1951 (4.5174~5.8416). The results indicate that low-energy N + implantation and mutation can be effective for increasing the virulence of Beauveria bassiana. (More)

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Paper citation in several formats:
Deng, X.; Tang, J.; Zhou, G. and Cao, J. (2019). Mutation of Beauveria Bassiana Using Low-energy N+ Implantation and Selection of a High Virulence Strain. In The Second International Conference on Materials Chemistry and Environmental Protection - MEEP; ISBN 978-989-758-360-5, SciTePress, pages 19-24. DOI: 10.5220/0008184800190024

@conference{meep19,
author={Xiaojun Deng. and Jian Tang. and Guoying Zhou. and Jizhao Cao.},
title={Mutation of Beauveria Bassiana Using Low-energy N+ Implantation and Selection of a High Virulence Strain},
booktitle={The Second International Conference on Materials Chemistry and Environmental Protection - MEEP},
year={2019},
pages={19-24},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0008184800190024},
isbn={978-989-758-360-5},
}

TY - CONF

JO - The Second International Conference on Materials Chemistry and Environmental Protection - MEEP
TI - Mutation of Beauveria Bassiana Using Low-energy N+ Implantation and Selection of a High Virulence Strain
SN - 978-989-758-360-5
AU - Deng, X.
AU - Tang, J.
AU - Zhou, G.
AU - Cao, J.
PY - 2019
SP - 19
EP - 24
DO - 10.5220/0008184800190024
PB - SciTePress