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Authors: Alessandro Giusti 1 ; Giorgio Corani 1 ; Luca Gambardella 2 ; Cristina Magli 3 and Luca Gianaroli 4

Affiliations: 1 SUPSI and University of Lugano, Switzerland ; 2 SUPSI and University of Lugan, Switzerland ; 3 International Institute for Reproductive Medicine, Switzerland ; 4 INFERGEN, Switzerland

ISBN: 978-989-674-018-4

Keyword(s): In vitro fertilization, Microscopy, Phase contrast, Hoffmann modulation contrast, Segmentation, Contouring, Autofocus.

Related Ontology Subjects/Areas/Topics: Applications and Services ; Artificial Intelligence ; Biomedical Engineering ; Biomedical Signal Processing ; Computer Vision, Visualization and Computer Graphics ; Data Manipulation ; Health Engineering and Technology Applications ; Human-Computer Interaction ; Medical Image Detection, Acquisition, Analysis and Processing ; Methodologies and Methods ; Neurocomputing ; Neurotechnology, Electronics and Informatics ; Pattern Recognition ; Physiological Computing Systems ; Sensor Networks ; Soft Computing

Abstract: Observation of ovocytes and zygotes plays an important role in In Vitro Fertilization procedures, and is usually perfomed by means of a microscope equipped with Hoffman Modulation Contrast optics, which produces images with a complex, side-lit, 3D-like appearance. Our algorithm operates on a Z-stack of such images taken at different focal planes, and simultaneously identifies: a) a repeatable, meaningful focal plane corresponding to the cell's equator line, and b) the external contour of the cell. As the cell is a thick stucture with respect to the microscope depth of field, the two problems are nontrivial and deeply related. Our algorithm is also robust to other structures, clutter and artefacts affecting the images and lying at varying focal planes. We describe implementation details, applications and experimental results of our technique.

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Paper citation in several formats:
Giusti A.; Corani G.; Gambardella L.; Magli C.; Gianaroli L. and (2010). SIMULTANEOUS FOCUSING AND CONTOURING OF HUMAN ZYGOTES FOR IN VITRO FERTILIZATION.In Proceedings of the Third International Conference on Bio-inspired Systems and Signal Processing - Volume 1: BIOSIGNALS, (BIOSTEC 2010) ISBN 978-989-674-018-4, pages 151-158. DOI: 10.5220/0002592501510158

@conference{biosignals10,
author={Alessandro Giusti and Giorgio Corani and Luca Gambardella and Cristina Magli and Luca Gianaroli},
title={SIMULTANEOUS FOCUSING AND CONTOURING OF HUMAN ZYGOTES FOR IN VITRO FERTILIZATION},
booktitle={Proceedings of the Third International Conference on Bio-inspired Systems and Signal Processing - Volume 1: BIOSIGNALS, (BIOSTEC 2010)},
year={2010},
pages={151-158},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0002592501510158},
isbn={978-989-674-018-4},
}

TY - CONF

JO - Proceedings of the Third International Conference on Bio-inspired Systems and Signal Processing - Volume 1: BIOSIGNALS, (BIOSTEC 2010)
TI - SIMULTANEOUS FOCUSING AND CONTOURING OF HUMAN ZYGOTES FOR IN VITRO FERTILIZATION
SN - 978-989-674-018-4
AU - Giusti, A.
AU - Corani, G.
AU - Gambardella, L.
AU - Magli, C.
AU - Gianaroli, L.
PY - 2010
SP - 151
EP - 158
DO - 10.5220/0002592501510158

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