Authors:
Lisa Savitri
1
;
Willy Sandhika
2
and
Agung Dwi Wahyu Widodo
3
Affiliations:
1
Department of Immunology, Postgraduate School and Universitas Airlannga, Indonesia
;
2
Department of Pathology, Faculty of Medicine and Universitas Airlangga, Indonesia
;
3
Department of Microbiology Clinic, Faculty of Medicine, RSUD Dr. Soetomo and Surabaya, Indonesia
Keyword(s):
Caspase-3 expression, Escherichia coli ESBL, Klebsiella pneumoniae carbapenemase
Abstract:
Sepsis is the leading cause of death in the world. Sepsis patients with Extended Spectrum β-lactamase (ESBL)-producing bacterial infections were 57.4% Escherichia coli, 21.35% Enterobacter sp, and 21.3% Klebsiella sp. Caspase-3 is the most important caspase effector responsible for morphological and biological changes in apoptotic cells. This type of research is true experimental with a post-test only control group design, using one control rat group and two groups of rats infected with E. coli Extended Spectrum β-lactamase (ESBL) and Klebsiela pneumoniae carbapenemase (KPC) for 24 hours to find out the different expression of caspase-3 in the spleen and liver of those infected rats. Expression of caspase-3 was observed by staining the spleen and liver with caspase-3 p12 subunit antibody. Cells expressing caspase-3 were counted under the light microscope. The results showed that caspase-3 expression in the KPC infected spleen group was 65.25±12.69%, whereas E. coli ESBL was 33.75±3.8
62%. This is thought to be influenced by the presence of antigen differences between the two bacteria, thus the possibility of apoptosis in lymphocyte cells caused by KPC would be higher when compared with those infected with E. coli ESBL. Caspase-3 liver expression in the KPC group had a value of 58.75±4.031%, while the E. coli ESBL infected was 48.75±6.292%. It may be affected by differences in soluble factors of both bacteria, thus the possibility of apoptosis in hepatocyte-induced cells by KPC will be higher when compared with those that are E. coli ESBL infected.
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