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Authors: Ahmad Shobrun Jamil 1 ; Siti Rofida 2 ; Dian Priyani 2 ; Wahidatin Nabila 2 and Evi Wulandari 2

Affiliations: 1 University of Muhammadiyah Malang, Indonesia ; 2 Department of Pharmacy, Faculty of Health Sciences, University of Muhammadiyah Malang Jalan Bendungan Sutami 188

Keyword(s): antimikroba, fraksi etanol, Limonia acidissima, skrining fitokimia

Abstract: The aim of this study is to determine the content of secondary metabolites ethanol fraction of fruit peel of Limonia acidissima qualitatively and test the activity of antimicrobial on Escherichia coli, Staphylococcus aureus, and Candida albicans. Phytochemical screening was carried out by running for separating the extract in silica gel by eluent n-hexane: ethyl acetate. The Thin Layer Chromatography (TLC) plate results then sprayed with a stain viewer; Alkaloid stain viewer: Dragendorff reagent; Terpenoids: Anisaldehyde-sulfuric acid reagents. The plate is heated to a temperature of 100°C; Flavonoids: H2 SO4 10% reagents; Polyphenols and tannins: FeCl 3 reagents 10%; Anthraquinone: 10% KOH reagent in methanol. The antimicrobial test was carried out using disk diffusion methods with concentrations of 50 mg/ml, 75 mg/ml and 100 mg/ml with control of nystatin and chloramphenicol. Phytochemical screening results of ethanol fraction Limonia acidissima can be detected in the content of al kaloids, terpenoids, anthraquinones, and saponins. Antimicrobial test results obtained data that the ethanol fraction Limonia acidissima has an increasing inhibitory activity of 50 mg/ml, 75 mg/ml, and 100 mg/ml. (More)

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Paper citation in several formats:
Jamil, A.; Rofida, S.; Priyani, D.; Nabila, W. and Wulandari, E. (2020). Phytochemical Screening and Antimicrobial Activity of Limonia acidissima Ethanol Extract Against Microbes from Clinical Isolates. In Proceedings of the 2nd Health Science International Conference - HSIC; ISBN 978-989-758-462-6, SciTePress, pages 152-156. DOI: 10.5220/0009126001520156

@conference{hsic20,
author={Ahmad Shobrun Jamil. and Siti Rofida. and Dian Priyani. and Wahidatin Nabila. and Evi Wulandari.},
title={Phytochemical Screening and Antimicrobial Activity of Limonia acidissima Ethanol Extract Against Microbes from Clinical Isolates},
booktitle={Proceedings of the 2nd Health Science International Conference - HSIC},
year={2020},
pages={152-156},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0009126001520156},
isbn={978-989-758-462-6},
}

TY - CONF

JO - Proceedings of the 2nd Health Science International Conference - HSIC
TI - Phytochemical Screening and Antimicrobial Activity of Limonia acidissima Ethanol Extract Against Microbes from Clinical Isolates
SN - 978-989-758-462-6
AU - Jamil, A.
AU - Rofida, S.
AU - Priyani, D.
AU - Nabila, W.
AU - Wulandari, E.
PY - 2020
SP - 152
EP - 156
DO - 10.5220/0009126001520156
PB - SciTePress

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