Extraction of Intrinsic Fluorescence in Fluorescence Imaging of Turbid Tissues

Gennadi Saiko; Alexandre Douplik

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Paper

Extraction of Intrinsic Fluorescence in Fluorescence Imaging of Turbid Tissues

Topics: Image Processing Methods; Quantitative Bioimaging

In Proceedings of the 13th International Joint Conference on Biomedical Engineering Systems and Technologies - Volume 2: BIOSTEC, 123-129, 2020 , Valletta, Malta

Authors: Gennadi Saiko ¹ and Alexandre Douplik ²

Affiliations: ¹ Swift Medical Inc, 1 Richmond St W, Toronto, Canada ; ² Department of Physics, Ryerson University, Toronto, Canada

Keyword(s): Fluorescence, Fluorescence Imaging, Intrinsic Fluorescence, Turbid Tissues.

Abstract: Interpretation of tissue fluorescence spectra can be complicated due to interplay with tissue optics. We have developed a photon propagation approach for correction of fluorescence on absorption in two realistic scenarios: when fluorophores are located a) on the surface of the turbid tissue and b) in a layer inside the turbid tissue. The approach takes into account the diffuse reflection of the tissue at excitation and emission wavelengths and does not require any precise measurement of optical properties (e.g., coefficient of absorption). The approach can be implemented using an inexpensive imaging setup and can be used in any setting.

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Paper citation in several formats:

Saiko, G. and Douplik, A. (2020). Extraction of Intrinsic Fluorescence in Fluorescence Imaging of Turbid Tissues. In Proceedings of the 13th International Joint Conference on Biomedical Engineering Systems and Technologies (BIOSTEC 2020) - BIOIMAGING; ISBN 978-989-758-398-8; ISSN 2184-4305, SciTePress, pages 123-129. DOI: 10.5220/0008919401230129

@conference{bioimaging20,
author={Gennadi Saiko. and Alexandre Douplik.},
title={Extraction of Intrinsic Fluorescence in Fluorescence Imaging of Turbid Tissues},
booktitle={Proceedings of the 13th International Joint Conference on Biomedical Engineering Systems and Technologies (BIOSTEC 2020) - BIOIMAGING},
year={2020},
pages={123-129},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0008919401230129},
isbn={978-989-758-398-8},
issn={2184-4305},
}

TY - CONF

JO - Proceedings of the 13th International Joint Conference on Biomedical Engineering Systems and Technologies (BIOSTEC 2020) - BIOIMAGING
TI - Extraction of Intrinsic Fluorescence in Fluorescence Imaging of Turbid Tissues
SN - 978-989-758-398-8
IS - 2184-4305
AU - Saiko, G.
AU - Douplik, A.
PY - 2020
SP - 123
EP - 129
DO - 10.5220/0008919401230129
PB - SciTePress