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Authors: M. Scaramuzza ; A. Ferrario ; E. Pasqualotto ; G. Rosati ; A. De Toni ; M. Quarta ; A. Paccagnella and C. Reggiani

Affiliation: University of Padova, Italy

Keyword(s): Lactate oxidase, Amperometric detection, Low-cost, Clinical applications, Food processing applications.

Related Ontology Subjects/Areas/Topics: Biomedical Engineering ; Biomedical Equipment ; Biomedical Instruments and Devices ; Biomedical Sensors ; Microelectronics ; Spectroscopic Applications

Abstract: In this work we present the preliminary resulting measurements of an enzyme-based biosensor for the amperometric detection of lactic acid (LA). The sensor is based on low-cost gold electrodes on polymeric substrate. The redox catalytic enzyme used for analyte amperometric detection is lactate oxidase (LOx) from Pediococcus sp. This enzyme has been immobilized over electrodes surfaces by direct adsorption methodologies. Analysis of the enzyme-modified electrodes have been carried out by means of Electrochemical Impedance Spectroscopy (EIS) and with the development of an equivalent electrical model, in order to improve the adsorption process. Biosensors performance have been evaluated with Cyclic Voltammetry (CVM) measurements in different lactic acid solutions with concentrations from 1 M up to 300 mM. The lactate sensitivity of this disposable biosensor results in about 6.24 µA mM-1 cm-2.

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Paper citation in several formats:
Scaramuzza, M.; Ferrario, A.; Pasqualotto, E.; Rosati, G.; De Toni, A.; Quarta, M.; Paccagnella, A. and Reggiani, C. (2012). LOW-COST ENZYME-BASED BIOSENSOR FOR LACTIC ACID AMPEROMETRIC DETECTION - Electrical Modeling and Validation for Clinical and Food Processing Applications. In Proceedings of the International Conference on Biomedical Electronics and Devices (BIOSTEC 2012) - BIODEVICES; ISBN 978-989-8425-91-1; ISSN 2184-4305, SciTePress, pages 380-383. DOI: 10.5220/0003867603800383

@conference{biodevices12,
author={M. Scaramuzza. and A. Ferrario. and E. Pasqualotto. and G. Rosati. and A. {De Toni}. and M. Quarta. and A. Paccagnella. and C. Reggiani.},
title={LOW-COST ENZYME-BASED BIOSENSOR FOR LACTIC ACID AMPEROMETRIC DETECTION - Electrical Modeling and Validation for Clinical and Food Processing Applications},
booktitle={Proceedings of the International Conference on Biomedical Electronics and Devices (BIOSTEC 2012) - BIODEVICES},
year={2012},
pages={380-383},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0003867603800383},
isbn={978-989-8425-91-1},
issn={2184-4305},
}

TY - CONF

JO - Proceedings of the International Conference on Biomedical Electronics and Devices (BIOSTEC 2012) - BIODEVICES
TI - LOW-COST ENZYME-BASED BIOSENSOR FOR LACTIC ACID AMPEROMETRIC DETECTION - Electrical Modeling and Validation for Clinical and Food Processing Applications
SN - 978-989-8425-91-1
IS - 2184-4305
AU - Scaramuzza, M.
AU - Ferrario, A.
AU - Pasqualotto, E.
AU - Rosati, G.
AU - De Toni, A.
AU - Quarta, M.
AU - Paccagnella, A.
AU - Reggiani, C.
PY - 2012
SP - 380
EP - 383
DO - 10.5220/0003867603800383
PB - SciTePress

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